TC-H 106
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
TC-H 106是I型组蛋白去乙酰化酶的抑制剂，对HDAC1、HDAC2、HDAC3和HDAC8的IC50值分别为150、760、370和5000 nM[1]。
去乙酰化酶（HADC）可以除去组蛋白中ε-N-乙酰赖氨酸上的乙酰基，使组蛋白更紧密地包裹住DNA，阻止转录。 I型组蛋白去乙酰化酶包含HDAC1、2、3和8 [1]。
在Friedreich共济失调患者的淋巴母细胞系中，2 μM 的TC-H 106延长组蛋白H3的乙酰化[1]。在HeLa细胞中，TC-H 106抑制HDACs，IC50值为1.3 μM。TC-H 106抑制重组HDAC3 / NcoR2和重组HDAC1，IC50值分别为0.79和0.24 μM[2]。
在小鼠frataxin基因的第一个内含子中携带（GAA）230重复的KIKI小鼠中，TC-H 106增加frataxin mRNA的含量。染色质中靠近GAA重复的组蛋白H3和H4乙酰化增加。这些结果导致KIKI小鼠的神经系统和心脏中frataxin恢复到正常水平[3]。
参考文献：
[1]. Chou CJ, Herman D, Gottesfeld JM. Pimelic diphenylamide 106 is a slow, tight-binding inhibitor of class I histone deacetylases. J Biol Chem, 2008, 283(51): 35402-35409.
[2]. Xu C, Soragni E, Chou CJ, et al. Chemical probes identify a role for histone deacetylase 3 in Friedreich's ataxia gene silencing. Chem Biol, 2009, 16(9): 980-989.
[3]. Rai M, Soragni E, Jenssen K, et al. HDAC inhibitors correct frataxin deficiency in a Friedreich ataxia mouse model. PLoS One, 2008, 3(4): e1958.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 339.43 |
| Cas No. | 937039-45-7 |
| Formula | C20H25N3O2 |
| Solubility | insoluble in H2O; ≥15.2 mg/mL in DMSO; ≥7.46 mg/mL in EtOH with ultrasonic |
| Chemical Name | N'-(2-aminophenyl)-N-(4-methylphenyl)heptanediamide |
| SDF | Download SDF |
| Canonical SMILES | CC1=CC=C(C=C1)NC(=O)CCCCCC(=O)NC2=CC=CC=C2N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
