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T7 Tag Peptide

现货
Catalog No.
A6008
多肽序列-MASMTGGQQMG
组合的产品项目
规格价格库存 数量
5mg
¥ 1,290.00
现货
25mg
¥ 5,180.00
现货

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Background

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems [1].


References:
1. Studier, F.W. and Moffatt, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-30.

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
M.Wt1098.27
FormulaC41H71N13O16S3
SynonymsH-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-OH
Solubility≥54.9mg/mL in DMSO, <2.5 mg/mL in EtOH, ≥7.14 mg/mL in H2O with ultrasonic
Chemical NameT7 Tag
SDFDownload SDF
Canonical SMILESCC(C(C(=O)NCC(=O)NCC(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCSC)C(=O)NCC(=O)O)NC(=O)C(CCSC)NC(=O)C(CO)NC(=O)C(C)NC(=O)C(CCSC)N)O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

生物活性

描述 T7 Tag Peptide是来源于T7主要衣壳蛋白的含11个氨基酸的多肽。
靶点 anti-T7-Tag antibody          
IC50            

质量控制

化学结构

T7 Tag Peptide