Sulfo-NHS-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-NHS-Biotin (N-羟基磺酸基琥珀生物素)是一种胺反应性的生物素化试剂。最常见的胺反应性生物素化试剂可分为两种，N-羟基琥珀酰亚胺（NHS）酯和羧酸盐。通常，NHS酯-生物素化试剂包含反应性的NHS环结构，可以与蛋白或其它分子羰基上的胺反应，通过亲核攻击形成稳定的酰胺键，并释放NHS基团。由于NHS-biotin在水溶液中的不溶性，通过在NHS环结构上加入一个带负电荷的磺酸基，从而获得NHS-biotin的水溶性类似物sulfo-NHS-biotin。Sulfo-NHS-Biotin可以直接加入到反应性水溶液中，而不需要提前溶解在有机溶剂中。
参考文献：
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- 1. Le Wang, Song Huang, et al. "Bio-inspirited Three-Dimensional Hierarchical Micro/Nano-Structured Microdevice for Enhanced Capture and Effective Release of Circulating Tumor Cells." Chemical Engineering Journal. Volume 435, Part 1, 1 May 2022, 134762.
- 2. Jun Lin, Xiaoxiao Jiang, et al. "Hepatokine Pregnancy Zone Protein Governs the Diet‐Induced Thermogenesis Through Activating Brown Adipose Tissue." Adv Sci (Weinh). 2021 Nov;8(21):e2101991. PMID: 34514733
- 3. Nathan J. Dwarshuis, Hannah W. Song, et al. "Functionalized microcarriers improve T cell manufacturing by facilitating migratory memory T cell production and increasing CD4/CD8 ratio." bioRxiv. 2019 May 23.
- 4. Lambert Yue, Christine Sam, et al. "Antibody microarray and immunoblotting analyses of the EGF signaling phosphorylation network in human A431 epidermoid carcinoma cells." Clinical Proteomics & Bioinformatics. 2017.
• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the N-terminal-amine
• Solubility—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only
| Physical Appearance | A solid |
| Storage | Desiccate at -20°C该产品在溶液中不稳定，建议现配现用 |
| M.Wt | 443.4 |
| Cas No. | 119616-38-5 |
| Formula | C14H18N3NaO8S2 |
| Synonyms | Sulfo-NHS Biotin |
| Solubility | ≥22.17 mg/mL in DMSO; insoluble in EtOH; ≥59.9 mg/mL in H2O with ultrasonic |
| Chemical Name | sodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 生物素化方法 [1]: | |
|
样品 |
THUMPa 蛋白 |
|
溶解方法 |
可以溶于水中。 |
|
反应条件 |
2 mM, 室温反应30 min |
|
应用 |
用含50 mM NaCl 的浓度为50 mM的K2HPO4/KH2PO4 缓冲液 （pH 7.5）将Sulfo-NHS-biotin溶解成 2 mM 的溶液。它可以立即用于分离试验中对浓度为1.66 nmol 的THUMPa蛋白进行标记。在室温下孵育30 min完成标记反应。反应完成后，样品用50 mM K2HPO4/KH2PO4 缓冲液（pH 7.5，含50 mM NaCl）透析15分钟。与蛋白质共价结合的生物素 可以用链霉素结合实验检测。 |
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References: [1]. Guillaume Gabant, Julie Augier and Jean Armengaud. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J. Mass Spectrom. 2008; 43: 360–370. |
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| 描述 | Sulfo-NHS-Biotin是一种水溶性的生物素化试剂，将生物素共价结合在伯胺上。 | |||||
| 靶点 | ||||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
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