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Sulfo-NHS-Biotin

现货
Catalog No.
A8001
水溶性的生物素化试剂
组合的产品项目
规格价格库存 数量
100mg
¥ 750.00
Ship with 10-15 days
1g
¥ 3,800.00
Ship with 10-15 days

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Background

Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates.  Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

文献引用

1. Nathan J. Dwarshuis, Hannah W. Song, et al. "Functionalized microcarriers improve T cell manufacturing by facilitating migratory memory T cell production and increasing CD4/CD8 ratio." bioRxiv. 2019 May 23.
2. Lambert Yue, Christine Sam, et al. "Antibody microarray and immunoblotting analyses of the EGF signaling phosphorylation network in human A431 epidermoid carcinoma cells." Clinical Proteomics & Bioinformatics.2017.

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the N-terminal-amine
• Solubility—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
该产品在溶液中不稳定,建议现配现用
M.Wt443.4
Cas No.119616-38-5
FormulaC14H18N3NaO8S2
SynonymsSulfo-NHS Biotin
Solubility≥22.17mg/mL in DMSO, <2.97 mg/mL in EtOH, ≥59.9 mg/mL in H2O with ultrasonic
Chemical Namesodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
SDFDownload SDF
Canonical SMILESC1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

生物素化方法 [1]:

样品

THUMPa 蛋白

溶解方法

可以溶于水中。

反应条件

2mM, 室温反应30min

应用

用含50 mM NaCl 的浓度为50 mM的K2HPO4/KH2PO4 缓冲液 (pH 7.5)将Sulfo-NHS-biotin溶解成 2 mM 的溶液。它可以立即用于分离试验中对浓度为1.66 nmol 的THUMPa蛋白进行标记。在室温下孵育30 min完成标记反应。反应完成后,样品用50mM K2HPO4/KH2PO4 缓冲液(pH 7.5,含50 mM NaCl)透析15分钟。与蛋白质共价结合的生物素 可以用链霉素结合实验检测。

References:

[1]. Guillaume Gabant, Julie Augier and Jean Armengaud. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J. Mass Spectrom. 2008; 43: 360–370.

生物活性

描述 Sulfo-NHS-Biotin是一种水溶性的生物素化试剂,将生物素共价结合在伯胺上。
靶点            
IC50            

质量控制

化学结构

Sulfo-NHS-Biotin

相关生物数据

Sulfo-NHS-Biotin

相关生物数据

Sulfo-NHS-Biotin

相关生物数据

Sulfo-NHS-Biotin