Cy5 carboxylic acid
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred. To labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In the soma of SNc DA neurons, to examine whether cyamemazine accumulates in the soma of SNc DA neurons, ovoid neurons were patch clamped and filled with the Sulfo-Cy5 carboxylic acid. [1].
Reference:
[1] Tucker, K. R.; Block, E.R.; Levitan, E.S. Action potentials and amphetamine release antipsychotic drug from dopamine neuron synaptic VMAT vesicles. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(32), E4485–E4494.
Storage | Store at -20°C |
Chemical Name | (2E)-1-(5-carboxypentyl)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate |
SDF | Download SDF |
Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 646 | 最大发射光波 (nm) | 662 |
消光系数 (M-1cm-1) | 271000 | 量子产量 | 0.28 |
CF260 | 0.04 | CF280 | 0.04 |