Cy3 azide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In vitro, sulfo-Cy3 azide was used for fluorescent microscopy staining. Human U87MG glioblastoma cells that overexpress uPAR were just stained by Cy3-AE105 conjugate [1].
Reference:
[1] Sun, L. Gai, Y. Anderson, C.J.; Zeng, D. Highly-efficient and versatile fluorous-tagged Cu (I)-catalyzed azide–alkyne cycloaddition ligand for preparing bioconjugates. Chemical Communications, 2015, 51, 17072–17075.
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| M.Wt | 752.88 |
| Formula | C34H45N6NaO8S2 |
| Solubility | ≥10 mg/mL in DMSO; ≥16.67 mg/mL in EtOH; ≥16.67 mg/mL in H2O |
| Chemical Name | (2E)-1-[6-(3-azidopropylamino)-6-oxohexyl]-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)prop-2-enylidene]indole-5-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=C3C(C4=C(N3CCCCCC(=O)NCCCN=[N+]=[N-])C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 最大激发光波 (nm) | 563 | 最大发射光波 (nm) | 584 |
| 消光系数 (M-1cm-1) | 162000 | 量子产量 | 0.1 |
| CF260 | 0.03 | CF280 | 0.06 |
质量控制和MSDS
- 批次:
化学结构
相关生物数据
