Streptozocin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Antibiotic and antitumor agent. Alkylates DNA and induces diabetes mellitus via reduction of nicotinamide adenine dinucleotide in pancreatic β-cells in vivo.
- 1. Ying Deng, Wenyi Zhu, et al. "Exendin-4 promotes bone formation in diabetic states via HDAC1-Wnt/β-catenin axis." Biochem Biophys Res Commun. 2021 Mar 12;544:8-14. PMID: 33516884
- 2. Ivana Stojanovic´, Katarina Šavikin, et al. "Pomegranate peel extract ameliorates autoimmunity in animal models of multiple sclerosis and type 1 diabetes." Journal of Functional Foods 35 (2017) 522–530.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 265.22 |
Cas No. | 18883-66-4 |
Formula | C8H15N3O7 |
Solubility | ≥10.3 mg/mL in DMSO; ≥26.5 mg/mL in EtOH with gentle warming; ≥53.2 mg/mL in H2O |
Chemical Name | 1-methyl-1-nitroso-3-[2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]urea |
SDF | Download SDF |
Canonical SMILES | O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(N(N=O)C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[1] | |
Cell lines |
A murine pancreatic β cell line, INS-1 |
Reaction Conditions |
15 or 30 mM streptozocin for 1 h incubation |
Applications |
Higher rates of apoptosis, as compared to necrosis, were observed when cells were exposed to 15 mM streptozocin for 1 h followed by a 24 h recovery period. Higher doses of streptozocin (30 mM) caused the cells to undergo necrosis (22%) as well as apoptosis (17%). Streptozotocin at low doses induced apoptosis and at high doses caused necrosis in INS-1 cells. |
Animal experiment:[2] | |
Animal models |
Male adult Holtzman rats |
Dosage form |
50, 65 or 100 mg/kg A single intravenous injection |
Applications |
Streptozotocin (65 mg/kg) resulted in rapid degranulation of β cells, accumulation of glycogen in the proximal convoluted tubules of the kidney, as well as development of cataracts in 4 months after streptozotocin treatment. At a higher dose of 100 mg/kg, streptozotocin produced lesions in the exocrine cells of the pancreas, and led to persistence of small, possibly secretory, granules in the Golgi zone of β cells in diabetic rats. Streptozotocin is often used to induce diabetes mellitus in experimental animals. |
Note |
The technical data provided above is for reference only. |
References: 1. Saini KS, Thompson C, Winterford CM, et al. Streptozotocin at low doses induces apoptosis and at high doses causes necrosis in a murine pancreatic beta cell line, INS-1. Biochemistry and Molecular Biology International, 1996, 39(6): 1229-1236. 2. Arison RN, Ciaccio EI, Glitzer MS, et al. Light and electron microscopy of lesions in rats rendered diabetic with streptozotocin. Diabetes, 1967, 16(1): 51-56. |
质量控制和MSDS
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