SLIGKV-NH2
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50值：蛋白酶激活受体2（PAR2）激动剂，IC50值为10.4 μM。
SLIGKV-NH2是一种蛋白酶激活受体2（PAR2）激动剂。PARs是一组存在于几种细胞类型的G蛋白偶联受体。目前，包括PAR1到4在内的四种PAR成员已被识别、克隆及指定。PAR2在呼吸道及胃肠道表达。PAR2活化与各种细胞和组织内的炎症反应密切相关。经鉴定，PAR2可诱导蛋白酶激活，因此导致全身性低血压[1]。
体外实验：经报道，通过诱导PAR2表达，SLIGKV-NH2（PAR2激活肽）可通过人体内支气管上皮细胞，轻度增强粘蛋白分泌。根据这项研究，与经反向氨基酸序列的对照肽处理的细胞相比，细胞暴露于100和1000 M浓度的SLIGKV-NH2 30分钟可导致粘蛋白分泌微弱但统计上显着的增加。另外，SLIGKV-NH2可加速细胞周期进程，且刺激HepG2细胞生长[1, 2]。
体内实验：体内研究PAR2激动剂诱导收缩反应的能力。发现小鼠PAR2激活(SLIGRL-NH2)和人类PAR2激活(SLIGKV-NH2)肽在豚鼠胆囊中引发浓度依赖性的收缩反应[3]。
临床试验：体内合成PAR2激活肽SLIGKV-NH2及其反向序列对照肽VKGILS-NH2，以验证人体内PAR2活化可引发血管舒张的假设。该项研究结果表明，在前臂阻力血管中，SLIGKV-NH2引发剂量依赖性扩张，而VKGILS-NH2没有显著效果[4]。
参考文献：
[1] Lin KW, Park J, Crews AL, Li YH, Adler KB. Protease-activated receptor-2 (PAR-2) is a weak enhancer of mucin secretion by human bronchial epithelial cells in vitro. Int J Biochem Cell B. 2008. 40: 137988.
[2]Xie L, Zheng Y, Li X, Zhao JY, Chen XY, Chen L, Zhou J, Hai O and Li F. Enhanced proliferation of human hepatoma cells by PAR-2 agonists via the ERK/AP-1 pathway. Oncol Rep. 2012.28: 1665-72.
[3] Tognetto M, Trevisani M, Maggiore B, Navarra G, Turini A, Guerrini R, Bunnett NW, Geppetti P and Harrison S. Evidence that PAR-1 and PAR-2 mediate prostanoid-dependent contraction in isolated guinea-pig gallbladder. Br.J.Pharmacol. 2000.131: 689-94.
[4] Robin J, Kharbanda R, Mclean P, Campbell R, Vallance P. Protease-activated receptor 2–mediated vasodilatation in humans in vivo, role of nitric oxide and prostanoids. Circulation. 2003;107:954-959.
| Physical Appearance | White lyophilised solid |
| Storage | Desiccate at -20°C |
| M.Wt | 614.78 |
| Cas No. | 190383-13-2 |
| Formula | C28H54N8O7 |
| Solubility | ≥13.48 mg/mL in EtOH with gentle warming and ultrasonic; ≥62.9 mg/mL in DMSO; ≥66.2 mg/mL in H2O |
| Chemical Name | (2S)-6-amino-2-[[2-[[(2S,3S)-2-[[(2S)-2-[[(2S)-2-amino-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-methylpentanoyl]amino]acetyl]amino]-N-[(2S)-1-amino-3-methyl-1-oxobutan-2-yl]hexanamide |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C(C(=O)NCC(=O)NC(CCCCN)C(=O)NC(C(C)C)C(=O)N)NC(=O)C(CC(C)C)NC(=O)C(CO)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
