SKI II
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SKI II是一种鞘氨醇激酶抑制剂,IC50值为0.5 μM[1].
SKI II对SK具有选择性,对人类蛋白激酶ERK2\PKC-I和脂质激酶PI3K无抑制能力.SKI II在SK的ATP结合位点上不是竞争性抑制剂.SKI II在完整的MDA-MB-231细胞中也可抑制内源SK[1].
作为一种SK抑制剂,SKI II可防止SK催化鞘氨醇-1-磷酸(S1P)生成.对S1P生成的抑制可导致对细胞增殖的抑制,从而诱导癌细胞凋亡.SKI II在T-24人类膀胱癌细胞\MCF-7人类乳腺癌细胞及MCF-7细胞的亚系MCF-7/VP具有细胞毒性,IC50值分别为4.6 μM\1.2 μM和0.9 μM[1].
参考文献：
[1] French KJ, Schrecengost RS, Lee BD, Zhuang Y, Smith SN, Eberly JL, Yun JK, Smith CD. Discovery and evaluation of inhibitors of human sphingosine kinase. Cancer Res. 2003 Sep 15;63(18):5962-9.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 302.78 |
| Cas No. | 312636-16-1 |
| Formula | C15H11ClN2OS |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥15.15 mg/mL in DMSO |
| Chemical Name | 4-[[4-(4-chlorophenyl)-1,3-thiazol-2-yl]amino]phenol |
| SDF | Download SDF |
| Canonical SMILES | C1=CC(=CC=C1C2=CSC(=N2)NC3=CC=C(C=C3)O)Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
