SKF 96365 hydrochloride
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SKF 96365 hydrochloride is an inhibitor of store-operated calcium entry (SOCE). Also, it inhibits transient receptor potential cation channels (TRPCs) and voltage-gated sodium current   .
Store-operated calcium entry (SOCE) increases cytosolic Ca2+ and induces the entry of extracellular calcium. TRPC channels are divided into store-operated and receptor-operated channels and play a critical role in brain development  .
SKF 96365 hydrochloride is a SOCE inhibitor. In MPP+ stressed PC12 cells, SKF-96365 (10, 50 μM) significantly prevented nuclear damage, decreased LDH release, increased cell viability and inhibited apoptosis. Also, SKF-96365 inhibited Homer1-mediated endoplasmic reticulum (ER) Ca2+ release and intracellular calcium overload induced by MPP+ injury . In rat ventricular myocytes, SKF-96365 inhibited INa in a frequency-dependent and use-dependent way . In Madin Darby canine kidney (MDCK) cells, SKF 96365 (25-100 μM) induced a robust [Ca2+]i transient in a dose-dependent way .
In rats, SKF-96365 significantly inhibited persistent spontaneous nociception induced by melittin and reduced hypersensitivity to both mechanical and thermal stimuli. These results suggested that SKF-96365-sensitive TRPC channels were involved in the processing of melittin-induced hypersensitivity and inflammatory pain .
. Chen T, Zhu J, Zhang C, et al. Protective effects of SKF-96365, a non-specific inhibitor of SOCE, against MPP+-induced cytotoxicity in PC12 cells: potential role of Homer1. PLoS One, 2013, 8(1): e55601.
. Ding J, Zhang JR, Wang Y, et al. Effects of a non-selective TRPC channel blocker, SKF-96365, on melittin-induced spontaneous persistent nociception and inflammatory pain hypersensitivity. Neurosci Bull, 2012, 28(2): 173-181.
. Chen KH, Liu H, Yang L, et al. SKF-96365 strongly inhibits voltage-gated sodium current in rat ventricular myocytes. Pflugers Arch, 2015, 467(6): 1227-1236.
. Jan CR, Ho CM, Wu SN, et al. Multiple effects of 1-[beta-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride (SKF 96365) on Ca2+ signaling in MDCK cells: depletion of thapsigargin-sensitive Ca2+ store followed by capacitative Ca2+ entry, activation of a direct Ca2+ entry, and inhibition of thapsigargin-induced capacitative Ca2+ entry. Naunyn Schmiedebergs Arch Pharmacol, 1999, 359(2): 92-101.
2. Wang Z, Wang Y, et al. "Transient receptor potential channel 1/4 reduces subarachnoid hemorrhage-induced early brain injury in rats via calcineurin-mediated NMDAR and NFAT dephosphorylation." Sci Rep. 2016 Sep 19;6:33577. PMID:27641617
|Storage||Store at RT|
|Solubility||≥40.3mg/mL in DMSO|
|Chemical Name||(S)-1-(2-(4-methoxyphenyl)-2-(3-(4-methoxyphenyl)propoxy)ethyl)-1H-imidazole hydrochloride|