[Ser25] Protein Kinase C (19-31)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
蛋白激酶C(19-31),化学式为C67H118N26O17,序列为H-ARG-PHE-ALA-ARG-LYS-GLY-SER-LEU-ARG-GLN-LYS-ASN-VAL-OH,分子量为1559.82。这种多肽是由PKCa(19-31)的伪底物调节结构域衍生而来的,第25位由丝氨酸取代了野生型PKCa的丙氨酸,常被用作蛋白激酶C的底物多肽来测定蛋白激酶C的活性。
蛋白激酶C也称为PKC,属于蛋白激酶家族,通过磷酸化其它蛋白质的丝氨酸和苏氨酸残基的羟基来调控蛋白的功能。研究表明,PKCa参与了许多不同的细胞过程,例如细胞粘附、细胞转化、细胞周期检测点和控制细胞体积。敲除小鼠的研究表明,该激酶可能是心脏收缩和肌细胞Ca2+处理的功能调节剂。
Figure1 Formula of [Ser25] Protein Kinase C (19-31)
Figure2 Signaling pathway of Protein Kinase C
参考文献:
1. Mellor H, Parker PJ (1998). "The extended protein kinase C superfamily". Biochem. J.. 332 ( Pt 2): 281–92
2. Nishizuka Y (1995). "Protein kinase C and lipid signaling for sustained cellular responses" (abstract). FASEB J. 9 (7): 484–96.
3. Vicente Micol. Correlation between Protein Kinase C an Activity and Membrane Phase Behavior. Departamento de Bioquı´mica y Biologı´a Molecular
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1559.82 |
Cas No. | 136795-05-6 |
Formula | C67H118N26O17 |
Synonyms | H2N-Arg-Phe-Ala-Arg-Lys-Gly-Ser-Leu-Arg-Gln-Lys-Asn-Val-OH |
Solubility | insoluble in EtOH; ≥155.9 mg/mL in DMSO; ≥65.2 mg/mL in H2O |
Chemical Name | [Ser25] Protein Kinase C (19-31) |
SDF | Download SDF |
Canonical SMILES | CC(C)CC(C(=O)NC(CCCN=C(N)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCCCN)C(=O)NC(CC(=O)N)C(=O)NC(C(C)C)C(=O)O)NC(=O)C(CO)NC(=O)CNC(=O)C(CCCCN)NC(=O)C(CCCN=C(N)N)NC(=O)C(C)NC(=O)C(CC1=CC=CC=C1)NC(=O)C(CCCN=C(N)N)N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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