Sedanolide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sedanolide is a natural compound produced in edible umbelliferous plants, such as Celery seed oil [1].
In HepG2 and CaCo-2 cells, treatment with sedanolide (7-500 μM) for 24h showed no effect on cell viability. In HepG2 cells cultured in sedanolide-free medium, sedanolide (500 μM) treatment for 72h decreased cell viability. Pretreatment with sedanolide (100 μM) for 24 h and exposement to either H2O2 or tBOOH did not exhibit statistically significant difference in viability from controls. In HepG2 following 24-h incubation with 500 μM sedanolide, a significant increase in DNA strand breaks was observed. Sedanolide did not modulate H2O2- and tBOOH-induced DNA damage. Sedanolide was relatively nontoxic to cells in culture [1]. Sedanolide (SN) possesses antioxidant effects. In human liver cancer (J5) cells, treatment with sedanolide suppressed J5 cell viability by inducing autophagy. Sedanolide decreased protein expression levels of phosphoinositide 3-kinase (PI3K)-I, mammalian target of rapamycin (mTOR) and Akt and increased PI3K-III, LC3-II and Beclin-1 protein levels. Sedanolide increased the cytosolic phosphorylation of inhibitor of kappa B (IκB) and nuclear p65 and the DNA-binding activity of NF-κB. Sedanolide induced J5 cell autophagy by regulating PI3K, p53 and NF-κB autophagy-associated signaling pathways in J5 cells [2]. Sedanolide (100 μg/ml) inhibited cyclooxygenases-1 and -2 at 250 pg/ml and blocked topoisomerase-I and-II activity [3].
References:
[1] Woods J A, Jewell C, O'Brien N M. Sedanolide, a natural phthalide from celery seed oil: effect on hydrogen peroxide and tert-butyl hydroperoxide-induced toxicity in HepG2 and CaCo-2 human cell lines[J]. In Vitro & Molecular Toxicology: A Journal of Basic and Applied Research, 2001, 14(3): 233-240.
[2] Hsieh S L, Chen C T, Wang J J, et al. Sedanolide induces autophagy through the PI3K, p53 and NF-κB signaling pathways in human liver cancer cells[J]. International journal of oncology, 2015, 47(6): 2240-2246
[3] Momin R A, Nair M G. Antioxidant, cyclooxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. seeds[J]. Phytomedicine, 2002, 9(4): 312-318.
Storage | Store at -20°C |
M.Wt | 194.3 |
Cas No. | 6415-59-4 |
Formula | C12H18O2 |
Solubility | insoluble in H2O; ≥52.8 mg/mL in EtOH; ≥6.65 mg/mL in DMSO |
Chemical Name | 3-butyl-3a,4,5,6-tetrahydro-1(3H)-isobenzofuranone |
SDF | Download SDF |
Canonical SMILES | O=C1OC(CCCC)C2CCCC=C21 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |