Gossypol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Gossypol是来源于棉花茎、叶、种子和花芽的天然酚化合物。Gossypol分子量为518.55道尔顿,是一种黄色颜料。Gossypol最常见的毒性作用是损害男性和女性生殖系统,另一个重要的毒性作用是干扰免疫功能,降低动物抗感染并损害疫苗的效率[1]。
体外实验:在牛粒细胞中,用gossypol治疗以剂量依赖性方式降低hCG诱导的cAMP形成。Gossypol(12.5 μg/ml)抑制基础cAMP水平和孕酮分泌(2)。Gossypol(50和100 μg/ml)减少完成泳动过程的精子的百分比。当用5或10 μg/ml gossypol培养时,切割胚胎的发育减少(3)。在从BALB/c小鼠的淋巴结中分离的淋巴细胞中,gossypol以剂量依赖性方式显著抑制佛波醇酯和离子霉素刺激的小鼠淋巴细胞的增殖。Gossypol显著抑制T淋巴细胞亚群和B淋巴细胞亚群的淋巴细胞转化。此外,Gossypol可以以时间和剂量依赖的方式诱导淋巴细胞凋亡(4)。
在体实验:在雄性Sprague-Dawley大鼠中,腹腔注射gossypol(25 mg/kg)显著改变肝和血清γ-谷氨酰转移酶(GGT)和微粒体单加氧酶的活性(5)。在大鼠中,较低剂量的gossypol(15 mg/kg/天持续四周或30 mg/kg/天持续给药两周)引起肝脏中明显的形态学变化(6)。
参考文献:
Gadelha I C N, Fonseca N B S, Oloris S C S, et al. Gossypol toxicity from cottonseed products[J]. The Scientific World Journal, 2014, 2014.
Lin Y C, Coskun S, Sanbuissho A. Effects of gossypol on in vitro bovine oocyte maturation and steroidogenesis in bovine granulosa cells[J]. Theriogenology, 1994, 41(8): 1601-1611.
Brocas C, Rivera R M, Paula-Lopes F F, et al. Deleterious actions of gossypol on bovine spermatozoa, oocytes, and embryos[J]. Biology of reproduction, 1997, 57(4): 901-907.
Xu W, Xu L, Lu H, et al. The immunosuppressive effect of gossypol in mice is mediated by inhibition of lymphocyte proliferation and by induction of cell apoptosis[J]. Acta Pharmacologica Sinica, 2009, 30(5): 597-604.
Deoras D P, Young-Curtis P, Dalvi R R, et al. Effect of gossypol on hepatic and serum γ-glutamyltransferase activity in rats[J]. Veterinary research communications, 1997, 21(5): 317-323.
Ying W, Hai-Peng L. Hepatotoxicity of gossypol in rats[J]. Journal of ethnopharmacology, 1987, 20(1): 53-64.
2. Liang J, Chen C, et al. "Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis." Biomed Res Int. 2018 Dec 13;2018:3635485. PMID:30643801
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 518.55 |
Cas No. | 303-45-7 |
Formula | C30H30O8 |
Solubility | ≥25.95 mg/mL in DMSO; insoluble in H2O; ≥2.1 mg/mL in EtOH |
Chemical Name | 7-(8-formyl-1,6,7-trihydroxy-3-methyl-5-propan-2-ylnaphthalen-2-yl)-2,3,8-trihydroxy-6-methyl-4-propan-2-ylnaphthalene-1-carbaldehyde |
SDF | Download SDF |
Canonical SMILES | OC1=C(O)C(C=O)=C(C(O)=C(C2=C(C)C=C(C(C(C)C)=C(O)C(O)=C3C=O)C3=C2O)C(C)=C4)C4=C1C(C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | Gossypol is an inhibitor of platelet-activating factor (PAF). | |||||
Targets | PAF | |||||
IC50 |
质量控制和MSDS
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