BRD 7552
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BRD7552是胰腺十二指肠同源框1（PDX1）的诱导剂[1] [2]。
PDX1是包含同源结构域的转录因子家族的成员。它是一个关键的转录因子，对成熟β细胞的功能和胰腺发育至关重要[1]。PDX1是β细胞转分化的一个主调节转录因子[2]。
在人类细胞中，BRD7552通过表观遗传学改变PDX1启动子区域，上调PDX1的表达[3]。在基于细胞的表型筛选试验中，BRD7552被发现是PDX1的诱导剂，它被用来诱导PDX1的表达。在PANC-1细胞中，BRD7552上调PDX1的mRNA和蛋白质水平。BRD7552在PDX1启动子区域改变表观遗传标记，这与转录激活一致。在PANC-1细胞培养中，BRD7552部分补充PDX1，增强引入三种基因诱导的胰岛素表达[2]。在PANC-1细胞中，BRD7552处理9天，剂量依赖性增加胰岛素mRNA表达。在原代人胰岛细胞5个供体样本中的三个中，BRD7552治疗3或5天剂量依赖性地显著诱导PDX1，治疗5天后显著诱导胰岛素[1]。在人PANC-1导管细胞系中，BRD7552能够诱导PDX1的表达[4]。
目前没有可用的在体实验数据。
参考文献：
[1]. Yuan Y, Hartland K, Boskovic Z, et al. A small-molecule inducer of PDX1 expression identified by high-throughput screening[J]. Chemistry & biology, 2013, 20(12): 1513-1522.
[2]. Yuan Y. Small-Molecule Modulators of Pancreatic Ductal Cells: Histone Methyltransferases and β-Cell Transdifferentiation [D]. 2013.
[3]. Zhao Y. Developing Therapies with Functional Beta Cells to Treat Diabetes[J]. International Journal of Translational Science, 2015, 1: 41-66.
[4]. Fodor A, Cozma A, Karnieli E. Personalized epigenetic management of diabetes[J]. Personalized Medicine, 2015, 12(5): 497-514.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 711.63 |
| Cas No. | 1137359-47-7 |
| Formula | C33H33N3O15 |
| Solubility | ≥71.2 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | ethyl 4-(((((2R,3R,4S,5R,6S)-4,5-bis((benzo[d][1,3]dioxol-5-ylcarbamoyl)oxy)-2-(hydroxymethyl)-6-methoxytetrahydro-2H-pyran-3-yl)oxy)carbonyl)amino)benzoate |
| SDF | Download SDF |
| Canonical SMILES | O=C(OCC)C1=CC=C(NC(O[C@@H]2[C@@H](CO)O[C@H](OC)[C@H](OC(NC3=CC=C(OCO4)C4=C3)=O)[C@H]2OC(NC5=CC=C(OCO6)C6=C5)=O)=O)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
