EIPA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EIPA is a TRPP3 channel inhibitor with an IC50 value of 10.5 μM, EIPA also inhibits Sodium-hydrogen exchangers (NHE) and macropinocytosis[1].
In Chinese hamster ovary cells, EIPA is a Na+/Ca2+ exchange (NCX) inhibitor that inhibits ET-1-induced continuous increase of intracellular Ca2+ concentration. In Xenopus oocytes, EIPA can rapidly and reversibly block calcium ion-activated TRPP3 channel activation at -50mV and other membrane potentials[2].
References:
[1]. Dai X Q, et al. Inhibition of TRPP3 channel by MK-870 and analogs. Mol Pharmacol, 2007, 72(6): 1576-85.
[2]. Horinouchi T, Miyake Y, Nishiya T, et al. Functional Role of Na+/H+ Exchanger in Ca2+ Influx Mediated via Human Endothelin Type A Receptor Stably Expressed in Chinese Hamster Ovary Cells. Journal of Pharmacological Sciences, 2008, 107(4): 456-459.
- 1. Jia Wen, Xiuge Gao, et al. "Optimization of Tilmicosin-Loaded Nanostructured Lipid Carriers Using Orthogonal Design for Overcoming Oral Administration Obstacle." Pharmaceutics. 2021 Feb 25;13(3):303. PMID:33669090
- 2. Zhang Q, Yang H, et al. "Nanostructured lipid carriers with exceptional gastrointestinal stability and inhibition of P-gp efflux for improved oral delivery of tilmicosin." Colloids Surf B Biointerfaces. 2019 Nov 17:110649. PMID:31767412
- 3. Li Y, Yang B, et al. "Oral delivery of imatinib through galactosylated polymeric nanoparticles to explore the contribution of a saccharide ligand to absorption." Int J Pharm. 2019 Jul 9;568:118508. PMID:31299337
- 4. Liu W, Zhang L, et al. "Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells." Bone Res. 2018 Sep 11;6:27. PMID:30210899
- 5. Siu FY, Ye S, et al. "Galactosylated PLGA nanoparticles for the oral delivery of resveratrol: enhanced bioavailability and in vitro anti-inflammatory activity." Int J Nanomedicine. 2018 Jul 13;13:4133-4144. PMID:30038494
- 6. Gao W, Ye G, et al. "Transferrin receptor-targeted pH-sensitive micellar system for diminution of drug resistance and targetable delivery in multidrug-resistant breast cancer." Int J Nanomedicine. 2017 Feb 7;12:1047-1064. PMID:28223798
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 299.76 |
| Cas No. | 1154-25-2 |
| Formula | C11H8ClN7O |
| Solubility | Soluble in DMSO |
| Chemical Name | 3-amino-6-chloro-N-(diaminomethylene)-5-(ethyl(isopropyl)amino)pyrazine-2-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | ClC1=NC(C(/N=C(N)\N)=O)=C(N)N=C1N(CC)C(C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]: | |
|
细胞系 |
中国仓鼠卵巢细胞； 表达TRPP3的非洲爪蟾卵母细胞 |
|
溶解方法 |
在DMSO中的溶解度>10mM。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
中国仓鼠卵巢细胞用10μM EIPA处理30分钟； 用100 μM EIPA处理非洲爪蟾卵母细胞 |
|
应用 |
在中国仓鼠卵巢细胞中，EIPA是一个Na+/Ca2+ 交换(NCX)抑制剂，抑制ET-1诱导的细胞内Ca2+ 浓度持续增加([Ca2+]i)。在非洲爪蟾卵母细胞中，EIPA在-50mV以及其他膜电位下能够快速且可逆地阻断Ca2+激活的TRPP3通道活化。 |
|
References: [1] .Dai et al (2007) Inhibition of TRPP3 channel by amiloride and analogs. Mol.Pharmacol. 72 1576. PMID: 17804601. [2]. Horinouchi et al (2008) Functional role of Na+/H+ exchanger in Ca2+ influx mediated via human endothelin type A receptor stably expressed in Chinese hamster ovary cells. J.Pharm.Sci. 107 456. PMID: 18678984. |
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质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
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