Veratridine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 673.79 |
| Cas No. | 71-62-5 |
| Formula | C36H51NO11 |
| Solubility | <33.69mg/ml in DMSO |
| Chemical Name | (3S,4S,4aS,6aS,6bS,8R,8aS,9S,9aS,12S,15aS,15bR,16aR,16bR)-4,6b,8,8a,9,15b-hexahydroxy-9,12,16b-trimethyldocosahydro-1H-4,16a-epoxybenzo[4,5]indeno[1,2-h]pyrido[1,2-b]isoquinolin-3-yl 3,4-dimethoxybenzoate |
| SDF | Download SDF |
| Canonical SMILES | O[C@@]([C@@]1([C@H](CN2[C@H]3CC[C@H](C)C2)[C@]4([C@@]3(C)O)O)O)(C[C@H]4O)[C@H]5[C@@]([C@@](CC[C@@H]6OC(C(C=C7OC)=CC=C7OC)=O)(C)[C@@H]8CC5)(C1)O[C@@]86O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]: | |
|
细胞系 |
HCT-116结肠癌细胞(表达GFP-UBXN2A蛋白) |
|
溶解方法 |
在DMSO中的溶解度>10mM。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
20和40 μM, 24小时 |
|
应用 |
Veratridin是主要的UBXN2A增强子，以剂量依赖的方式增加UBXN2A蛋白水平。 |
| 动物实验[1]: | |
|
动物模型 |
C57Bl/6N小鼠(表达GFP-UBXN2A蛋白的HCT-116细胞注射入6-8周龄裸鼠雌性小鼠) |
|
剂量 |
0.125 mg/kg；腹膜内注射(IP)；28天 |
|
应用 |
Veratridine (VTD) 有效诱导UBXN2A蛋白水平，导致UBXN2A和mot-2依赖性的结肠癌细胞死亡。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。 |
|
References: [1]. Abdullah A, Sane S, Branick KA, et al. A plant alkaloid, veratridine, potentiates cancer chemosensitivity by UBXN2A-dependent inhibition of an oncoprotein, mortalin-2. Oncotarget. 2015 Sep 15;6(27):23561-81. | |
质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
