Autocamtide-2-related inhibitory peptide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50：作用于CaM激酶II为40 nM [1]。
Synthetic peptide AIP（autocamtide-2相关抑制肽）是autocamtide-2的一种非磷酸化类似物，被确认为一种高特异性的和强效的钙调蛋白依赖性蛋白激酶II（CaM激酶II，CaMKII）抑制剂。 CaMKII是一种丝氨酸/苏氨酸特异性蛋白激酶，由Ca2+/钙调蛋白调控。
体外：AIP（1 mM）完全抑制CaMKII的活性，但并不影响cAMP依赖性蛋白激酶、钙调蛋白依赖性蛋白激酶IV和蛋白激酶C。所述抑制是非竞争性的，通过结合到与外源性底物不同的自磷酸化位点引起作用。CaM Ⅱ自磷酸化的IC50为100 nM[1]。
体内：通过转基因表达AIP治疗的小鼠，可防止富含果糖饮食引起的心律失常、自发性收缩和自发性Ca2+释放[2]。AIP的内伏隔核（NAc）单一注射可剂量依赖性地增加大鼠对有害热和机械刺激的HWL（后爪缩回潜伏期）[3]。
临床试验：目前尚未进行临床研究。
参考文献：
[1] Ishida A1, Kameshita I, Okuno S, Kitani T, Fujisawa H. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995 Jul 26;212(3):806-12.
[2] Sommese L, Valverde CA, Blanco P, Castro MC, Rueda OV, Kaetzel M, Dedman J, Anderson ME, Mattiazzi A, Palomeque J. Ryanodine receptor phosphorylation by CaMKII promotes spontaneous Ca(2+) release events in a rodent model of early stage diabetes: The arrhythmogenic substrate. Int J Cardiol. 2016 Jan 1;202:394-406.
[3] Bian H, Yu LC. Intra-nucleus accumbens administration of the calcium/calmodulin-dependent protein kinase II inhibitor AIP induced antinociception in rats with mononeuropathy. Neurosci Lett. 2015 Jul 10;599:129-32.
| Physical Appearance | A crystalline solid |
| Storage | Desiccate at -20°C |
| M.Wt | 1497.77 |
| Cas No. | 167114-91-2 |
| Sequence | Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu |
| Formula | C64H116N22O19 |
| Solubility | ≥40.2 mg/mL in DMSO; insoluble in EtOH; ≥45.7 mg/mL in H2O |
| Chemical Name | (2S,5S,8S,11S,14S,17S)-8-(carboxymethyl)-17-((2S,5S,8S,11S,14S,17S,20S)-20,24-diamino-2-(3-amino-3-oxopropyl)-17-(4-aminobutyl)-5,8-bis(3-guanidinopropyl)-11-isobutyl-14-methyl-4,7,10,13,16,19-hexaoxo-3,6,9,12,15,18-hexaazatetracosanamido)-2-isobutyl-11-i |
| SDF | Download SDF |
| Canonical SMILES | [H]N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(O)=O)CC(C)C)=O)C)=O)CC(O)=O)=O)C(C)C)=O)C)=O)CCC(O)=O)=O)CCC(N)=O)=O)CCCNC(N)=N)=O)CCCNC(N)=N)=O)CC(C)C)=O)C)=O)CCCCN)=O)CCCCN |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
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化学结构
