8-CPT-2Me-cAMP, sodium salt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
8-CPT-2Me-cAMP, sodium salt是EPAC的选择性激动剂[1]。
环AMP鸟嘌呤核苷酸交换因子(EPACs)是cAMP的细胞内传感器,作为Ras GTPase同系物RAP1和RAP2的核苷酸交换因子而起作用[1]。
8-CPT-2Me-cAMP, sodium salt是EPAC的选择性激动剂。8-CPT-2Me-cAMP增加EPAC1对Rap1的激活。同时,微管相关蛋白MAP1A的轻链2(LC2)可增加该反应。在LC2和EPAC1转染细胞中,8-CPT-2Me-cAMP 增加细胞与层粘连蛋白的粘附[1]。在Jurkat T细胞中,8-CPT-2Me-cAMP(100 μM)激活Rap1,该反应不受PKA抑制剂H-89的影响[2]。在1-LN前列腺癌细胞中,8-CPT-2Me-cAMP以剂量依赖的方式增加Epac1、p-AktS473和p-AktT308。8-CPT-2Me-cAMP增加2-3倍的p-AktS473和AktS473激酶活性。8-CPT-2Me-cAMP也激活mTORC1和mTORC2[3]。
在人前列腺癌细胞中,8-CPT-2Me-cAMP增加p-cPLA2S505、COX-2和PGE2的水平。然而,COX-2、EP4或mTOR的抑制剂抑制该效应,减少Epac1诱导的蛋白和DNA合成。这些结果表明Epac1是促炎调制器,促进细胞增殖[4]。
参考文献:
[1]. Gupta M, Yarwood SJ. MAP1A light chain 2 interacts with exchange protein activated by cyclic AMP 1 (EPAC1) to enhance Rap1 GTPase activity and cell adhesion. J Biol Chem, 2005, 280(9): 8109-8116.
[2]. Fuld S, Borland G, Yarwood SJ. Elevation of cyclic AMP in Jurkat T-cells provokes distinct transcriptional responses through the protein kinase A (PKA) and exchange protein activated by cyclic AMP (EPAC) pathways. Exp Cell Res, 2005, 309(1): 161-173.
[3]. Misra UK, Pizzo SV. Upregulation of mTORC2 activation by the selective agonist of EPAC, 8-CPT-2Me-cAMP, in prostate cancer cells: assembly of a multiprotein signaling complex. J Cell Biochem, 2012, 113(5): 1488-1500.
[4]. Misra UK, Pizzo SV. Evidence for a pro-proliferative feedback loop in prostate cancer: the role of Epac1 and COX-2-dependent pathways. PLoS One, 2013, 8(4): e63150.
Physical Appearance | White lyophilised solid |
Storage | Desiccate at -20°C |
M.Wt | 507.82 |
Cas No. | 634207-53-7 |
Formula | C17H16ClN5O6PS·Na |
Solubility | <50.78mg/ml in H2O |
Chemical Name | sodium (4aS,6S,7S,7aS)-6-(6-amino-8-((4-chlorophenyl)thio)-9H-purin-9-yl)-7-methoxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide |
SDF | Download SDF |
Canonical SMILES | [H][C@]12COP(O[C@]1([H])[C@H](OC)[C@@H](N3C(SC4=CC=C(C=C4)Cl)=NC5=C3N=CN=C5N)O2)(O[Na])=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |