D-AP5
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
D-AP5 is a selective NMDA receptor antagonist (Kd = 1.4 μM), which can inhibit the glutamate binding site of NMDA receptors competitively[1]. D-AP5 causes sensorimotor disorders during spatial task, but gradually worsens as animals fail to learn. Rats treated with D-AP5 show a space-dependent delay-dependent defect in the delay-matched placement protocol of the water maze[2].
References:
[1]. Evans R H, Francis A A, Jones A W, et al. The effects of a series of ω-phosphonic α-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br. J. Pharmacol, 1982, 75(1): 65-75.
[2]. Morris R G, et al. N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. Eur J Neurosci, 2013, 37(5): 700-17.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 197.13 |
| Cas No. | 79055-68-8 |
| Formula | C5H12NO5P |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥28.1 mg/mL in H2O |
| Chemical Name | (R)-2-amino-5-phosphonopentanoic acid |
| SDF | Download SDF |
| Canonical SMILES | OP(O)(CCC[C@H](C(O)=O)N)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
新生儿切片，少年切片 |
|
溶解方法 |
该化合物在水中溶解为100 mM。若获取更高浓度的溶液，可在37℃下孵育10分钟，随后在超声波浴中摇匀。-20℃以下可储存数月。 |
|
反应条件 |
100 μM |
|
应用 |
在新生儿切片中，在100 μM D-AP5存在的情况下，5 Hz/3 min的刺激训练没有诱导EPSP的LTD。在青少年切片中，在100 μM D-AP5存在下，5 Hz/3 min的刺激训练没有诱导EPSP的LTD。在存在100 μM D-AP5的情况下，在感应协议期间将电压钳紧在-80 mV，阻止了mGluR-LTD的诱导。 |
| 动物实验 [2,3]: | |
|
动物模型 |
大鼠 |
|
给药剂量 |
0–50 mM |
|
应用 |
在大鼠中，通过渗透微型泵给药D-AP5（0-50 mM），以线性剂量依赖的方式损伤空间学习，与海马LTP的相应损伤高度相关。没有观察到能阻断LTP而不影响学习的D-AP5浓度。在急性海绵体中输注放射性标记的D-AP5，显示相对有限的扩散，并用于评估全组织海马药物浓度。D-AP5的慢性输注引起记忆的延迟依赖性损伤。 |
|
注意事项 |
由于实验环境的不同，实际溶解度可能与理论值略有不同，请测试室内所有化合物的溶解度。 |
|
References: [1]. Oliet S H R, Malenka R C, Nicoll R A. Two distinct forms of long-term depression coexist in CA1 hippocampal pyramidal cells[J]. Neuron, 1997, 18(6): 969-982. [2]. Davis S, Butcher S P, Morris R G. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro[J]. Journal of Neuroscience, 1992, 12(1): 21-34. [3]. Steele R J, Morris R G M. Delay‐dependent impairment of a matching‐to‐place task with chronic and intrahippocampal infusion of the NMDA‐antagonist D‐AP5[J]. Hippocampus, 1999, 9(2): 118-136. | |
质量控制和MSDS
- 批次:
化学结构
