Cyclosporin H
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cyclosporin H是一种有效的\选择性的以及竞争性的甲酰肽受体(FPR)拮抗剂,其IC50值为0.7 μM[1].
甲酰肽受体(FPR)属于G蛋白偶联受体,参与趋化作用并介导免疫细胞响应感染.
Cyclosporin H(CsH)是一种强效的FPR选择性拮抗剂.在人中性粒细胞中,Cyclosporin H抑制FMLP诱导的超氧阴离子(O2-)形成.CsH抑制FMLP(3 nM)与HL-60细胞膜结合,其IC50值与Ki值分别为0.7 μM和0.10 μM.CsH抑制FMLP(100 nM)诱导的GTP水解,其IC50值与Ki值分别为1.3 μM和0.79 μM.CSH还能抑制FMLP诱导的胞内Ca2+浓度\O2-形成以及β-葡糖醛酸酶释放的增加,其IC50值分别为0.23 μM\0.6 μM和2.2 μM,其Ki值分别为0.08 μM\0.24 μM和0.45 μM[1][2].在外周血嗜碱性粒细胞中,CsH(8 ~ 800 nM)呈浓度依赖性地竞争抑制FMLP诱导的白三烯C4以及组胺释放[3].使小鼠吸入过敏原,从小鼠支气管肺泡灌洗液中分离出嗜酸性粒细胞,Cyclosporin H与10 U/ml白细胞介素5联合用药呈浓度依赖性地促进细胞凋亡.Cyclosporin H与10 U/ml GM-CSF合用也能增加细胞凋亡,但其作用较弱[4].
参考文献:
[1]. Wenzel-Seifert K, Seifert R. Cyclosporin H is a potent and selective formyl peptide receptor antagonist. Comparison with N-t-butoxycarbonyl-L-phenylalanyl-L-leucyl-L-phenylalanyl-L- leucyl-L-phenylalanine and cyclosporins A, B, C, D, and E. J Immunol, 1993, 150(10): 4591-4599.
[2]. Wenzel-Seifert K, Grünbaum L, Seifert R. Differential inhibition of human neutrophil activation by cyclosporins A, D, and H. Cyclosporin H is a potent and effective inhibitor of formyl peptide-induced superoxide formation. J Immunol, 1991, 147(6): 1940-1946.
[3]. de Paulis A, Ciccarelli A, de Crescenzo G, et al. Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine. J Allergy Clin Immunol, 1996, 98(1): 152-164.
[4]. Kitagaki K, Nagai H, Hayashi S, et al. Facilitation of apoptosis by cyclosporins A and H, but not FK506 in mouse bronchial eosinophils. Eur J Pharmacol, 1997, 337(2-3): 283-289.
Physical Appearance | A white powder |
Storage | Store at -20°C |
M.Wt | 1202.61 |
Cas No. | 83602-39-5 |
Formula | C62H111N11O12 |
Solubility | ≥49.1 mg/mL in DMSO; ≥52.9 mg/mL in EtOH; insoluble in H2O |
Chemical Name | (3R,6S,9S,12R,13Z,15S,16Z,18S,21S,22Z,24S,30S,31E,33S)-30-ethyl-14,17,23,32-tetrahydroxy-33-((1R,2R,E)-1-hydroxy-2-methylhex-4-en-1-yl)-6,9,18,24-tetraisobutyl-3,21-diisopropyl-1,4,7,10,12,15,19,25,28-nonamethyl-1,4,7,10,13,16,19,22,25,28,31-undecaazacycl |
SDF | Download SDF |
Canonical SMILES | C/C([H])=C([H])/C[C@@]([C@](O)([H])[C@@](N1C)([H])/C(O)=N\[C@@](C(N(CC(N([C@@](/C(O)=N/[C@@](C(N([C@@](/C(O)=N/[C@@](/C(O)=N/[C@](C(N([C@@](C(N([C@@](C(N([C@](C1=O)([H])C(C)C)C)=O)([H])CC(C)C)C)=O)([H])CC(C)C)C)=O)([H])C)([H])C)([H])CC(C)C)C)=O)([H])C(C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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