ACSF
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ACSF通常用作CSF的替代物,可在脑片灌注过程中保护中间神经元 [1].
ACSF (人工脑脊液)可减少脑水肿的发生率,并进一步抑制脑细胞疾病。在神经外科领域,如颅内手术,ACSF常被用作冲洗液或灌洗液,或者当脑脊液流失时用作补充液。在科研中ACSF常用来维持pH平衡和维持组织氧输送[2,3]。
在体外急性大脑切片模型中研究神经功能的基本特征时,ACSF常用来维持氧的供给。在新皮层小鼠切片中,carbogenated ACSF处理可保持pH平衡和维持氧输送,而no-magnesium ACSF治疗的切片中出现癫痫样事件[1]。在保护神经元的靶向药物研究中,通常用ACSF处理小鼠作为对照来评估药效[3]。
也有研究指出在大鼠脑切片模型中,经ACSF(人CSF的替代物)处理可有力地促进CA1、CA3区和5层锥体神经元的自发放电[4]。
参考文献:
[1] Voss, L. J., S.A. George, and J.W. Sleigh, Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions. J Neurosci Methods, 2012. 204(2): p. 273-5.
[2] Liu, D. and F. Bao, Hydrogen peroxide administered into the rat spinal cord at the level elevated by contusion spinal cord injury oxidizes proteins, DNA and membrane phospholipids, and induces cell death: Attenuation by a metalloporphyrin. Neuroscience, 2015. 285: p. 81-96.
[3] Gruber, R. C., et al., Targeted GAS6 delivery to the CNS protects axons from damage during experimental autoimmune encephalomyelitis. J Neurosci, 2014. 34(49): p. 16320-35.
[4] Bjorefeldt, A. , et al., Human cerebrospinal fluid increases the excitability of pyramidal neurons in the in vitro brain slice. J Physiol, 2015. 593(1): p. 231-43.
Physical Appearance | Colourless liquid |
Storage | Store at RT |
M.Wt | 320.38 |
Formula | C20H20N2O2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |