740 Y-P
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
740 Y-P是一种细胞通透性的PI3K激活剂[1]。
PI3K（磷酸肌醇3-激酶，PI3Ks）是一种酶，在基础细胞代谢过程，如囊泡运输、细胞脱粒、细胞迁移和葡萄糖转运中起重要作用。据报道，PI3K的过表达与多种癌症相关[2]。
740 Y-P是一种有效的PI3K激活剂，在PI3K/AKT信号转导途径中起重要作用。在人黑色素瘤MNT-1细胞中，用20 μM 的740 Y-P处理24小时后激活PI3K，显著减少了蔗糖诱导的M6PR阳性空泡的数量[1]。在不含血清培养的小脑颗粒细胞中，740 Y-P通过与PI3K的调节亚基p85结合减少细胞死亡率，这与PI3K依赖性的Akt的磷酸化过程相关[3]。
参考文献：
[1]. Bin, B.H., et al., Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One, 2014. 9(8): p. e105965.
[2]. Kong, B., et al., A subset of metastatic pancreatic ductal adenocarcinomas depends quantitatively on oncogenic Kras/Mek/Erk-induced hyperactive mTOR signalling. Gut, 2015.
[3]. Williams, E.J. and P. Doherty, Evidence for and against a pivotal role of PI 3-kinase in a neuronal cell survival pathway. Mol Cell Neurosci, 1999. 13(4): p. 272-80.
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- 4. Bingkun Zhao, Qian Peng, et al. "Leonurine Promotes the Osteoblast Differentiation of Rat BMSCs by Activation of Autophagy via the PI3K/Akt/mTOR Pathway." Front Bioeng Biotechnol. 2021 Feb 23;9:615191. PMID: 33708763
- 5. Jeremy P Goering, Dona G Isai, et al. "SPECC1L-deficient primary mouse embryonic palatal mesenchyme cells show speed and directionality defects." Sci Rep. 2021 Jan 14;11(1):1452. PMID: 33446878
- 6. Yujie Zhao, Wenyan Guo, et al. "Repression of deoxynivalenol-triggered cytotoxicity and apoptosis by mannan/β-glucans from yeast cell wall: Involvement of autophagy and PI3K-AKT-mTOR signaling pathway." Int J Biol Macromol. 2020 Dec 1;164:1413-1421. PMID: 32735928
- 7. Xing W, Tan Y, et al. "Upregulated hepatokine fetuin B aggravates myocardial ischemia/reperfusion injury through inhibiting insulin signaling in diabetic mice." J Mol Cell Cardiol. 2020;S0022-2828(20)30057-2. PMID: 32147518
- 8. Jeremy P. Goering, Dona Greta Isai, et al. "SPECC1L-deficient palate mesenchyme cells show speed and directionality defect." bioRxiv. 2019 November 25.
- 9. Song Y, Liu H, et al. "Silencing of Peroxiredoxin 1 Inhibits the Proliferation of Esophageal Cancer Cells and Promotes Apoptosis by Inhibiting the Activity of the PI3K/AKT Pathway." Cancer Manag Res, 11, 10883-10890 2019 Dec 30. PMID: 31920397
- 10. Xiaolian Gu, Wenyan Guo, et al. "Deoxynivalenol-Induced Cytotoxicity and Apoptosis in IPEC-J2 Cells Through the Activation of Autophagy by Inhibiting PI3K-AKT-mTOR Signaling Pathway." ACS Omega. October 24, 2019.
- 11. Wu Y, Li Z, et al. "Tivantinib Hampers the Proliferation of Glioblastoma Cells via PI3K/Akt/Mammalian Target of Rapamycin (mTOR) Signaling." Med Sci Monit. 2019 Oct 2;25:7383-7390. PMID: 31575848
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- 13. Wang X, Qi X, et al. "Autophagy Benefits the Replication of Egg Drop Syndrome Virus in Duck Embryo Fibroblasts." Front Microbiol. 2018 May 29;9:1091. PMID: 29896171
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| Physical Appearance | A solid |
| Storage | Desiccate at -20°C |
| M.Wt | 3270.72 |
| Cas No. | 1236188-16-1 |
| Formula | C141H222N43O39PS3 |
| Solubility | ≥163.54 mg/mL in DMSO; insoluble in EtOH; ≥4.87 mg/mL in H2O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
人黑色素瘤细胞MNT-1 |
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制备方法 |
溶解度有限。若配制更高浓度的溶液，一般步骤如下：请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
|
反应条件 |
24 h |
|
实验结果 |
740 Y-P诱导M6PR阳性点的产生，其类似于蔗糖诱导的空泡。740 Y-P显著减少蔗糖引起的M6PR阳性空泡的数量。 |
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References: 1. Bin BH, Bhin J, Yang SH et al. Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One. 2014 Aug 29;9(8):e105965. |
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质量控制和MSDS
- 批次:
化学结构
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