BAPTA-AM
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BAPTA-AM是一种选择性钙螯合剂[1].
Ca2+是最普遍存在的功能广泛的胞内信号分子之一,可控制多个细胞过程,如神经递质释放\所有肌细胞类型的收缩和受精[4].
BAPTA-AM是一种选择性的细胞膜可透过的钙螯合剂.在人类白血病细胞系HL-60和U937中,BAPTA/AM(10 μM)可诱导核小体间的DNA剪切和经典凋亡形态.并且,BAPTA/AM可提高胞内Ca2+,下调c-jun[1].在牛嗜铬细胞中,APTA-AM(50 μM)可迅速可逆地抑制50%的Ca2+活化的K+(I(KCa))和电压门控的K+(I(K))[2].在HEK 293细胞中,BAPTA-AM可以浓度依赖的方式抑制hERG(Kv11.1)\hKv1.3和hKv1.5通道,IC50值分别为1.3\1.45和1.23 μM,这依赖于通道开放[3].
在swiss小鼠中,BAPTA-AM可抑制乙醇导致的运动刺激,并能逆转乙醇诱导的催眠作用.在雄性C57BL/6J小鼠中,BAPTA-AM可以剂量依赖的方式降低乙醇消耗[4].
参考文献:
[1]. Grant S, Freemerman AJ, Gregory PC, et al. Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM. Oncol Res, 1995, 7(7-8): 381-392.
[2]. Urbano FJ, Buo W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. Neuroreport, 1998, 9(15): 3403-3407.
[3]. Tang Q, Jin MW, Xiang JZ, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol, 2007, 74(11): 1596-1607.
[4]. Balio P, Monferrer L, Pastor R, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol, 2012, 234(2): 446-453.
- 1. Benjamin S. Roberts, Chelsea Q. Yang, et al. "Characterization of lipoprotein lipase storage vesicles in 3T3-L1 adipocytes." J Cell Sci. 2022 Mar 1;135(5):jcs258734. PMID:34382637
- 2. Li S, Lu D, et al. "Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free Ca(2+): Potential Mechanism of Pelvic Electrical Stimulation Therapy." Biomed Res Int. 2019 Apr 21;2019:7387803. PMID:31139648
- 3. Wang J, Yang C, et al. "T-2 Toxin Exposure Induces Apoptosis in TM3 Cells by Inhibiting Mammalian Target of Rapamycin/Serine/Threonine Protein Kinase(mTORC2/AKT) to Promote Ca(2+)Production." Int J Mol Sci. 2018 Oct 27;19(11). pii: E3360. PMID:30373220
- 4. Wang Y, Lu S, et al. "Sonic hedgehog induces GLT-1 degradation via PKC delta to suppress its transporter activities." Neuroscience. 2017 Oct 6;365:217-225. PMID:28993237
Storage | Desiccate at -20°C |
M.Wt | 764.68 |
Cas No. | 126150-97-8 |
Formula | C34H40N2O18 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥16.3 mg/mL in DMSO with gentle warming |
SDF | Download SDF |
Canonical SMILES | O=C(CN(CC(OCOC(C)=O)=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)OCOC(C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1, 2]: | |
细胞系 |
人白血病细胞系HL-60和U937,牛嗜铬细胞 |
溶解方法 |
该化合物在DMSO中的溶解度大于16.3 mg/mL。若获取更高浓度的溶液,可在37℃下孵育10分钟,随后在超声波浴中摇匀。-20℃以下可储存数月。 |
反应条件 |
10 μM,50 μM,6 h |
应用 |
在人白血病细胞系HL-60和U937中,BAPTA/AM(10 μM)诱导产生经典的凋亡形态,BAPTA/AM(50 μM)诱导产生一些非典型特征,例如细胞肿胀及染色质聚集。BAPTA-AM(50 μM)的灌注引起IKCa和IK快速(<60s)及可逆阻滞(~50%)。用BAPTA/AM(50 μM,30分钟)或用BAPTA的非通透活性形式(在移液管溶液中10 mM)进行预孵育,永久阻断IKCa。在BAPTA加载或BAPTA-AM预培养后,BAPTA-AM超灌注(50 μM)阻断IK(~53%)。 |
动物实验 [3]: | |
动物模型 |
Swiss (RjOrl)小鼠,雄性C57BL/6J小鼠 |
给药剂量 |
0–10 mg/kg,30 min |
应用 |
在Swiss (RjOrl)小鼠中,用BAPTA-AM(0-10 mg/kg,30分钟)预处理在不改变基础运动的条件下阻止了乙醇产生的运动刺激。BAPTA-AM逆转乙醇诱导的催眠作用。在黑暗喝酒方法之后,给予雄性C57BL/6J小鼠20%v/v乙醇、自来水或0.1%甜味水。BAPTA-AM预处理(0-5 mg/kg)以剂量依赖性方式降低乙醇消耗,而对水和甜水摄入不受影响。 |
注意事项 |
由于实验环境的不同,实际溶解度可能与理论值略有不同,请测试室内所有化合物的溶解度。 |
References: [1]. Grant S, Freemerman AJ, Gregory PC, et al. Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM. Oncol Res, 1995, 7(7-8): 381-392. [2]. Urbano FJ, Buo W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. Neuroreport, 1998, 9(15): 3403-3407. [3] Balio P, Monferrer L, Pastor R, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol, 2012, 234(2): 446-453. |
Description | BAPTA-AM是一种选择性的细胞膜可透过的钙螯合剂. | |||||
靶点 | ||||||
IC50 |
质量控制和MSDS
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