Caspofungin Acetate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspofungin acetate（MK-0991）是一种新的具有最小毒性的抗真菌剂，抑制1,3-β-D-葡聚糖的合成，1,3-β-D-葡聚糖是真菌细胞壁的一个重要组成部分[1]。
体外实验：单剂量的Caspofungin孵育烟曲霉菌影响相同比例的顶端和肩胛下分支细胞长达72小时。Caspofungin杀死烟曲霉菌丝中活性中心细胞的新细胞壁合成[1]。Caspofungin acetate不可逆地抑制酶1,3-β-D-葡聚糖合酶，防止葡聚糖聚合物的形成，破坏真菌细胞壁的完整性。Caspofungin acetate的消除半衰期为9-10小时[2]。Caspofungin对曲霉以及各种念珠菌具有活性[3]。Caspofungin acetate对白色念珠菌和热带假丝酵母分离株的生长动力学研究显示，在0.06至1 μg/ml（是MIC 的0.25至4倍）的浓度范围内，Caspofungin acetate在3至7小时内表现出杀真菌活性，活力降低99％[4]。Caspofungin acetate对假丝酵母属物种的90％最小抑制浓度如下：C. albicans 0.5 μg/mL（范围0.25-0.5）、C. glabrata 1.0 μg/mL（范围0.25-2.0）、C. tropicalis 1.0 μg/mL（范围0.25-1.0）、C. parapsilosis 0.5 μg/mL（范围，0.25-1.0）和C. krusei 2.0 μg/mL（范围，0.5-2.0）[4]。
临床试验：在128名念珠菌性食管炎患者的试验中，caspofungin acetate 50或70 mg/天（85％）的临床反应率高于amphotericin B 0.5 mg/kg/天（67％）[2]。
参考文献：
[1]. Bowman J C, Hicks P S, Kurtz M B, et al. The antifungal echinocandin caspofungin acetate kills growing cells of Aspergillus fumigatus in vitro[J]. Antimicrobial agents and chemotherapy, 2002, 46(9): 3001-3012.
[2]. Hoang A. Caspofungin acetate: an antifungal agent[J]. American journal of health-system pharmacy, 2001, 58(13): 1206-1214.
[3]. Pacetti S A, Gelone S P. Caspofungin acetate for treatment of invasive fungal infections[J]. Annals of Pharmacotherapy, 2003, 37(1): 90-98.
[4]. Bartizal K, Gill C J, Abruzzo G K, et al. In vitro preclinical evaluation studies with the echinocandin antifungal MK-0991 (L-743,872)[J]. Antimicrobial Agents and Chemotherapy, 1997, 41(11): 2326-2332.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 1213.42 |
| Cas No. | 179463-17-3 |
| Formula | C56H96N10O19 |
| Solubility | ≥60.67 mg/mL in DMSO; insoluble in EtOH; ≥21.5 mg/mL in H2O |
| Chemical Name | acetic acid compound with (Z)-N-((2R,6S,7Z,9S,11R,12S,13E,14aS,15S,20S,21Z,23S,24Z,25aS)-20-((R)-3-amino-1-hydroxypropyl)-12-((2-aminoethyl)amino)-23-((1S,2S)-1,2-dihydroxy-2-(4-hydroxyphenyl)ethyl)-2,8,11,14,15,22,25-heptahydroxy-6-((R)-1-hydroxyethyl)-5 |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)CC(C)CCCCCCCCC(=O)NC1CC(C(NC(=O)C2C(CCN2C(=O)C(NC(=O)C(NC(=O)C3CC(CN3C(=O)C(NC1=O)C(C)O)O)C(C(C4=CC=C(C=C4)O)O)O)C(CCN)O)O)NCCN)O.CC(=O)O.CC(=O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
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细胞系 |
烟曲霉细胞 |
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溶解方法 |
该化合物在DMSO中的溶解度 > 10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
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反应时间 |
32 μg/ml 处理6小时; 或2 μg/ml处理12小时 |
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应用 |
Caspofungin抑制细胞壁β-1,3-葡聚糖的合成，其引发对几丁质合成的补偿性刺激。Caspofungin诱导烟曲霉的形态学变化。此外，Caspofungin处理烟曲霉诱导ChsG依赖性几丁质合成的上调和富含几丁质的小菌落的形成。 |
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References: 1Walker, L. A., Lee, K. K., Munro, C. A. and Gow, N. A. (2015) Caspofungin Treatment of Aspergillus fumigatus Results in ChsG-Dependent Upregulation of Chitin Synthesis and the Formation of Chitin-Rich Microcolonies. Antimicrob Agents Chemother. 59, 5932-5941 |
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质量控制和MSDS
- 批次:
化学结构
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