Plerixafor 8HCl (AMD3100 8HCl)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Plerixafor 8HCl(AMD3100 8HCl)是一种有效的CXCL12介导的趋化作用和G蛋白偶联趋化因子受体(CXCR4)的选择性拮抗剂,IC50值分别为5.7和44 nM[1].相比其他趋化因子受体,包括LTB4 CCR1\CCR2b\CCR4\CCR5\CCR7和CXCR3等,Plerixafor 8HCl对CXCR4具有高选择性[2].
Plerixafor 8HCl可抑制I-SDF-1配体与表达CXCR4的CCRF–CEM T淋巴母细胞的结合.Plerixafor 8HCl可阻断CXCR4激活\SDF-1介导的钙流和SDF-1介导的趋化作用,IC50值分别为27.3\572和51 nM[2].
参考文献：
[1] Zabel BA1, Wang Y, Lewén S, Berahovich RD, Penfold ME, Zhang P, Powers J, Summers BC, Miao Z, Zhao B, Jalili A, Janowska-Wieczorek A, Jaen JC, Schall TJ. Elucidation of CXCR7-mediated signaling events and inhibition of CXCR4-mediated tumor cell transendothelial migration by CXCR7 ligands. J Immunol. 2009 Sep 1;183(5):3204-11. doi: 10.4049/jimmunol.0900269. Epub 2009 Jul 29.
[2] Fricker SP1, Anastassov V, Cox J, Darkes MC, Grujic O, Idzan SR, Labrecque J, Lau G, Mosi RM, Nelson KL, Qin L, Santucci Z, Wong RS. Characterization of the molecular pharmacology of AMD3100: a specific antagonist of the G-protein coupledchemokine receptor, CXCR4. Biochem Pharmacol. 2006 Aug 28;72(5):588-96.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 794.47 |
| Cas No. | 155148-31-5 |
| Formula | C28H62Cl8N8 |
| Solubility | ≥155.4 mg/mL in H2O; insoluble in DMSO; insoluble in EtOH |
| Chemical Name | 1-[[4-(1,4,8,11-tetrazacyclotetradec-1-ylmethyl)phenyl]methyl]-1,4,8,11-tetrazacyclotetradecane;octahydrochloride |
| SDF | Download SDF |
| Canonical SMILES | C1CNCCNCCCN(CCNC1)CC2=CC=C(C=C2)CN3CCCNCCNCCCNCC3.Cl.Cl.Cl.Cl.Cl.Cl.Cl.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]： | |
|
细胞系 |
骨髓单核细胞（BMMCs） |
|
溶解方法 |
溶解度有限。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
10 μM |
|
应用 |
在BMMCs中，AMD3100能够中和SDF-1α诱导的破骨细胞形成。AMD3100还可以降低SDF-1α增强的破骨细胞特异性蛋白表达。 |
| 动物实验[2]: | |
|
动物模型 |
8-10周龄的无特定病原体雌性C57BL/6小鼠 |
|
剂量 |
每天3 mg/kg，腹腔注射 |
|
应用 |
给药CXCR4拮抗剂AMD3100降低了与活化CD25+ T细胞上表达的白细胞介素-2受体特异性结合的示踪剂的摄取量，摄取量降低了2.8倍，表明在癌症治疗中活化T淋巴细胞的浸润依赖于CXCR4。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。 |
|
References: [1]. Dong Y, Liu H, Zhang X, et al. Inhibition of SDF-1α/CXCR4 signalling in subchondral bone attenuates post-traumatic osteoarthritis[J]. International Journal of Molecular Sciences, 2016, 17(6): 943. [2]. Hartimath S V, Draghiciu O, van de Wall S, et al. Noninvasive monitoring of cancer therapy induced activated T cells using [18F] FB-IL-2 PET imaging[J]. OncoImmunology, 2017, 6(1): e1248014. |
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质量控制和MSDS
- 批次:
化学结构
