p-Cresyl sulfate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
P-Cresol（对甲酚）是一种与蛋白相结合的尿毒症保留溶质[1]。
在非糖尿病血液透析患者中，游离对甲酚被认为是一个心血管风险因素。在糖尿病患者中，游离对甲酚和总对甲酚的水平明显更高。在用血液透析进行治疗的病人中，游离对甲酚和总对甲酚的水平比用血液透析滤过（hemodiafiltration）治疗的病人中更高。单因素Cox比例风险分析表明，游离对甲酚的浓度与心血管疾病（CVD）显著相关。相当多的含有高浓度游离对甲酚的病人都有新的致命性或非致命性心血管事件发生。除此之外，游离对甲酚还可以抑制细胞增殖，它抑制了26%的培养内皮细胞的增殖，但不影响细胞活力。而且，对甲酚以10、25和50 μg/ml的剂量处理时分别减少了19%、28%和40%的内皮损伤修复[1][2]。
参考文献：
1. Meijers B K I, Bammens B, De Moor B, et al. Free p-cresol is associated with cardiovascular disease in hemodialysis patients. Kidney international, 2008, 73(10): 1174-1180.
2. Dou L, Bertrand E, Cerini C, et al. The uremic solutes p-cresol and indoxyl sulfate inhibit endothelial proliferation and wound repair. Kidney international, 2004, 65(2): 442-451.
- 1. Yuanyuan Shi, Huajun Tian, et al. "Improved Dialysis Removal of Protein-Bound Uraemic Toxins with a Combined Displacement and Adsorption Technique." Blood Purif. 2022;51(6):548-558. PMID:34515053
- 2. Chih-Yu Yang, Ting-Wen Chen, et al. "Synbiotics Alleviate the Gut Indole Load and Dysbiosis in Chronic Kidney Disease." Cells. 2021 Jan 9;10(1):114. PMID:33435396
- 3. Tien-Hung Huang, Hon-Kan Yip, et al. "P-cresyl sulfate causes mitochondrial hyperfusion in H9C2 cardiomyoblasts." J Cell Mol Med. 2020 Aug;24(15):8379-8390. PMID:32639656
- 4. Van Buren J, Prasse C, et al. "Ring-Cleavage Products Produced during the Initial Phase of Oxidative Treatment of Alkyl-Substituted Aromatic Compounds." Environ Sci Technol. 2020;54(13):8352-8361. PMID:32519538
- 5. Shi Y, Tian H, et al. "Removal of Protein-Bound Uremic Toxins by Liposome-Supported Peritoneal Dialysis." Perit Dial Int. 2019 Nov-Dec;39(6):509-518. PMID:31690700
- 6. Potts DM, Peterson DG. "Identification of small molecule flavor compounds that contribute to the somatosensory attributes of bovine milk products." Food Chem. 2019 Oct 1;294:27-34. PMID:31126463
- 7. Shi Y, Zhang Y, et al. "Improved dialytic removal of protein-bound uremic toxins by intravenous lipid emulsion in chronic kidney disease rats." Nephrol Dial Transplant. 2019 May 9. pii: gfz079. PMID:31071223
- 8. Shi Y, Tian H, et al. "Effect of Ionic Strength, pH and Chemical Displacers on the Percentage Protein Binding of Protein-Bound Uremic Toxins." Blood Purif. 2018 Dec 18:1-10. PMID:30562731
- 9. Shi Y, Wang Y, et al. "Increasing the removal of protein-bound uremic toxins by liposome-supported hemodialysis."Artif Organs. 2018 Oct 30. PMID:30375673
| Physical Appearance | A solid |
| Storage | Store at -20°C该产品在溶液中不稳定，建议现配现用 |
| M.Wt | 188.20 |
| Cas No. | 3233-58-7 |
| Formula | C7H8O4S |
| Solubility | insoluble in EtOH; ≥30.1 mg/mL in DMSO; ≥50 mg/mL in H2O |
| Chemical Name | p-tolyl hydrogen sulfate |
| SDF | Download SDF |
| Canonical SMILES | CC1=CC=C(OS(O)(=O)=O)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验: [1] | |
|
细胞系 |
人脐静脉内皮细胞 |
|
制备方法 |
溶解度有限，若配制更高浓度的溶液，一般步骤如下：请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
|
反应条件 |
24 h |
|
实验结果 |
在含有和不含HAS的条件下，10 ug/mL、25 ug/mL和50 ug/mL p-cresol分别将内皮细胞的增殖减少21％、38％和54％。没有HAS存在的条件下，用p-cresol处理的单层中的内皮伤口修复明显低于对照组培养基处理的细胞。10 μg/mL、25 μg/mL和50 μg/mL p-cresol分别将内皮伤口修复减少19％、28％和40％。使用HSA，仅50 μg/mL p-cresol明显阻断内皮伤口修复。 |
| 动物实验: [2] | |
|
动物模型 |
肾功能正常和减少的大鼠 |
|
给药剂量 |
静脉注射p-cresol (10 mg/kg) |
|
制备方法 |
溶于等渗盐水中 |
|
实验结果 |
在肾功能正常和降低的小鼠中注射p-cresol，并与在类似条件下获得的肌酐（60 mg/kg）的结果进行比较。在肾功能降低的大鼠中，与具有正常肾功能的大鼠相比，肾功能下降的大鼠中p-cresol的血清浓度显示出最小的下降。在肾功能正常的大鼠中，注射的p-cresol有21.0 ± 10.0％经尿排出。在肾衰竭的大鼠中，量为6.7 ± 7.5％。 |
|
注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。 |
|
References: 1. Dou L, Bertrand E, Cerini C, et al. The uremic solutes p-cresol and indoxyl sulfate inhibit endothelial proliferation and wound repair. Kidney international, 2004, 65(2): 442-451. 2. Lesaffer G, De Smet R, D'Heuvaert T et al. Comparative kinetics of the uremic toxin p-cresol versus creatinine in rats with and without renal failure. Kidney Int. 2003 Oct; 64(4):1365-73. |
|
质量控制和MSDS
- 批次:
化学结构
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