Bleomycin Sulfate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Bleomycin sulfate(BLENOXANE)是由链霉菌verticillus 菌株产生的具细胞毒性的糖肽类抗生素混合物。已知此组分通过逼近金属和协调双氧以产生活性氧，引起DNA单链和/或双链的断裂。
Bleomycin sulfate治疗通过抑制DNA和蛋白质合成，导致E.coli 细胞伸长和HeLa细胞肿大[ 1 ]。
此组分广泛应用于：与羟柔毛霉素联合用药治疗Hodgkin's淋巴瘤，睾丸癌、鳞状细胞癌、肺纤维化以及足底疣的治疗。例如，Bleomycin sulfate对劳氏肉瘤病毒诱发的小鼠腹水肉瘤也显示具有中等到显著的作用，特别是在F1杂种小鼠被当作宿主动物时[ 2 ]。此外，近期研究支持促炎细胞因子IL-18 和IL-1beta在Bleomycin sulfate诱导的人和小鼠肺损伤机制中的作用[ 3 ]。
参考文献：
1.Suzuki H, Nagai K, Yamaki H, Tanaka N, Umezawa H. Mechanism of action of bleomycin. Studies with the growing culture of bacterial and tumor cells. J Antibiot (Tokyo) 1968,21:379-386.
2.Takeuchi M, Yamamoto T. Effects of bleomycin on transplantable mouse tumors. J Antibiot (Tokyo) 1968,21:631-637.
3.Hoshino T, Okamoto M, Sakazaki Y, Kato S, Young HA, Aizawa H. Role of proinflammatory cytokines IL-18 and IL-1beta in bleomycin-induced lung injury in humans and mice. Am J Respir Cell Mol Biol 2009,41:661-670.
- 1. Jingjing Zhang, Jinxin Qiu, et al. "Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity." Nat Immunol. 2022 Feb;23(2):237-250. PMID: 35075279
- 2. Zhao K, Wang X, et al. "A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers." PLoS Biol. 2020;18(3):e3000666. PMID: 32203529
- 3. Thompson PJ, Shah A, et al. "Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes." Cell Metab. 2019 Feb 14. pii: S1550-4131(19)30021-X. PMID: 30799288
| Physical Appearance | A solid |
| Storage | Store at -20° C |
| M.Wt | 1512.6 |
| Cas No. | 9041-93-4 |
| Formula | C55H85N17O25S4 |
| Synonyms | Blenoxane;Bleo;Blexane |
| Solubility | ≥125 mg/mL in DMSO with gentle warming; insoluble in EtOH; ≥151.3 mg/mL in H2O with ultrasonic |
| Chemical Name | 3-[[2-[2-[2-[[(2R,3R)-2-[[(2R,3R,4S)-4-[[(2S)-2-[[6-amino-2-[(1S)-3-amino-1-[[(2R)-2,3-diamino-3-oxopropyl]amino]-3-oxopropyl]-5-methylpyrimidine-4-carbonyl]amino]-3-[(2S,3R,4R,5R,6R)-3-[(2R,3S,4S,5R,6R)-4-carbamoyloxy-3,5-dihydroxy-6-(hydroxymethyl)oxan- |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)OC3C(C(C(C(O3)CO)O)O)OC4C(C(C(C(O4)CO)O)OC4C(C(C(C(O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
ADIPO-P2细胞 |
|
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
|
反应条件 |
2.5 μg/mL；30分钟 |
|
实验结果 |
在ADENO-P2细胞中，Bleomycin Sulfate具有明显的致瘤作用。停药后，上述作用仍能维持至少10天。 |
| 动物实验 [2]: | |
|
动物模型 |
CD-1小鼠 |
|
给药剂量 |
5 mg/kg，2 ml/kg，气管内给药 |
|
实验结果 |
Bleomycin Sulfate促进炎症发展，导致严重肺纤维化，同时增加TGF-β1、Smad3和STAT1的量。 |
|
其它注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。 |
|
References: [1]. Paviolo NS, Quiroga IY, Castrogiovanni DC, Bianchi MS, Bolzán AD. Telomere instability is present in the progeny of mammalian cells exposed to bleomycin. Mutat Res. 2012 Jun 1;734(1-2):5-11. [2]. Shi K, Jiang J, Ma T, Xie J, Duan L, Chen R, Song P, Yu Z, Liu C, Zhu Q, Zheng J. Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-β, Smad3 and JAK-STAT pathway. Int J Clin Exp Med. 2014 Sep 15;7(9):2645-50. |
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| 描述 | Bleomycin sulfate是一种糖肽类抗生素，也是鳞状细胞癌(SCC)的抗癌剂，在UT-SCC-19A细胞中IC50值为4 nM。 | |||||
| 靶点 | UT-SCC-129 cells | |||||
| IC50 | 4 nM | |||||
质量控制和MSDS
- 批次:
化学结构
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