Cisplatin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cisplatin是一种高效的和广谱的化疗剂[1]。
Cisplatin是一种抗癌试剂，通过多种机制诱导细胞凋亡，有一定的副作用。传统机制是：cisplatin进入细胞，与DNA的鸟嘌呤碱基相互作用，形成DNA链内及链间交联，从而阻止DNA复制。该信息也可通过激活p53诱导细胞凋亡。Cisplatin也可引起ROS生成并增加脂质过氧化，进而引起细胞凋亡。此外，Cisplatin也可通过caspase依赖的途径诱导细胞凋亡。在耳蜗细胞中，Cisplatin治疗可导致caspases-3 和caspases-9的增加，引起耳蜗损害的副作用[1]。
通过ERK信号通路介导caspase-3依赖的细胞凋亡。用cisplatin处理耳蜗细胞，可导致caspases-3 和caspases-9表达水平，导致耳蜗损害[1]。
参考文献：
[1] Casares C, Ramírez-Camacho R, Trinidad A, et al. Reactive oxygen species in apoptosis induced by cisplatin: review of physiopathological mechanisms in animal models. European Archives of Oto-Rhino-Laryngology, 2012, 269(12): 2455-2459.
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| Physical Appearance | A solid |
| Storage | Store at RT建议粉末形式避光保存，溶液形式非常不稳定（需现配现用，且低温易析出），使用DMF溶解，DMSO会使顺铂失活。 |
| M.Wt | 300.05 |
| Cas No. | 15663-27-1 |
| Formula | Cl2H6N2Pt |
| Synonyms | CDDP |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥12.5 mg/mL in DMF |
| Chemical Name | azane;dichloroplatinum(2+) |
| SDF | Download SDF |
| Canonical SMILES | N.N.Cl[Pt+2]Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
L1210/0细胞 |
|
制备方法 |
在DMF中的溶解度大于12.5 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。 |
|
反应条件 |
0、0.5、1、2、4和8 μg/mL；2小时 |
|
实验结果 |
在低浓度下，Cisplatin诱导最少细胞死亡。在较高浓度下，细胞死亡明显，只有4%存活。于孵育10天后，存活的细胞开始生长并且成为群体中的主要细胞。 |
| 动物实验 [2]: | |
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动物模型 |
携带人卵巢癌OVCAR-3细胞异种移植物的裸小鼠 |
|
给药剂量 |
5 mg/kg，静脉注射；在第0天和第7天 |
|
实验结果 |
于第0天和第7天，给予Cisplatin (5 mg/kg) 抑制OVCAR-3细胞异种移植物生长，肿瘤生长抑制(GI) 为85.1%。 |
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其它注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。 |
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References: [1]. Sorenson CM, Eastman A. Mechanism of cis-diamminedichloroplatinum(II)-induced cytotoxicity: role of G2 arrest and DNA double-strand breaks. Cancer Res. 1988 Aug 15;48(16):4484-8. [2]. Molthoff CF, Pinedo HM, Schlüper HM, Rutgers DH, Boven E. Comparison of 131I-labelled anti-episialin 139H2 with cisplatin, cyclophosphamide or external-beam radiation for anti-tumor efficacy in human ovarian cancer xenografts. Int J Cancer. 1992 Apr 22;51(1):108-15. |
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| Description | Cisplatin是一种高效的和广谱的化疗剂。 | |||||
| 靶点 | ||||||
| IC50 | ||||||
质量控制和MSDS
- 批次：
化学结构
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