KY 02111
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
KY 02111是一种选择性的Wnt抑制剂,在β连接蛋白破坏复合体中靶向GSK3β和APC的下游[1]。
Wnt信号通路是一组由蛋白组成的信号传导通路,在将细胞外信号通过细胞表面受体传递到细胞内的过程中发挥重要作用。Wnt信号起初被认为是癌症发生的关键介质,但后来认识到它在胚胎发育中的功能,包括体轴形成、细胞命运规范、细胞增殖和细胞迁移,这些都是重要组织形成所必须的,包括骨、心脏和肌肉[2]。
KY 02111是一种有效的Wnt抑制剂,与已报道的Wnt抑制剂具有不同的选择性。在转染TCF受体质粒的IMR90-1 hiPSCs细胞中,KY 02111(1 μM)通过抑制经典的Wnt信号传导途径,以剂量依赖的方式显著减少萤光素酶的活性。在心肌细胞集落中,第30天时,KY 02111诱导近73%-85%的表达心肌标志物的IMR90-1 hiPSCs[1]。
参考文献:
[1]. Minami, I., et al., A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep, 2012. 2(5): p. 1448-60.
[2]. Stamatakou, E., M. Hoyos-Flight, and P.C. Salinas, Wnt Signalling Promotes Actin Dynamics during Axon Remodelling through the Actin-Binding Protein Eps8. PLoS One, 2015. 10(8): p. e0134976.
Physical Appearance | A solid |
Storage | Store at 4°C |
M.Wt | 376.86 |
Cas No. | 1118807-13-8 |
Formula | C18H17ClN2O3S |
Solubility | insoluble in H2O; ≥18.85 mg/mL in DMSO; ≥7.06 mg/mL in EtOH with gentle warming and ultrasonic |
Chemical Name | N-(6-chloro-1,3-benzothiazol-2-yl)-3-(3,4-dimethoxyphenyl)propanamide |
SDF | Download SDF |
Canonical SMILES | COC1=C(C=C(C=C1)CCC(=O)NC2=NC3=C(S2)C=C(C=C3)Cl)OC |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
IMR90-1 hiPSCs |
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
反应条件 |
10 μM;12或24小时 |
实验结果 |
在IMR90-1 hiPSCs中,KY02111 (10 μM) 下调经典Wnt信号途径的靶基因表达 (72.7%)。 |
References: [1]. Minami, I., et al., A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep, 2012. 2(5): p. 1448-60. |
描述 | KY02111是hPSCs分化成心肌细胞的促进剂和Wnt信号的抑制剂。 | |||||
靶点 | Wnt Signaling | |||||
IC50 |
质量控制和MSDS
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