Cy3 NHS ester (non-sulfonated)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cyanine是一种属于聚甲炔基的合成染料,仍用于工业以增加感光乳剂的感光范围(例如增加波长范围且使薄膜全色),最近用于生物技术领域.作为荧光染料,Cyanine已用于生物医学成像.根据它们的结构,cyanines能覆盖UV到IR的光谱范围.
Cyanine3 NHS ester可用作反应性染料,用于标记生物分子中的氨基,是Cy3 NHS ester的类似物.该试剂通常用于标记可溶性蛋白\多肽\寡核苷酸或DNA.Cyanine3 NHS ester需要使用有机共溶剂(例如DMF或DMSO)用于标记,然而,考虑到水溶性sulfo-Cyanine3 NHS ester在精细蛋白中的使用,此类共溶剂不是必需的.Cy3荧光位于橙色区域(~550 nm激发, ~570 nm发射),而Cy5是在红色区域发光(~650/670 nm),且在橙色区域吸收(~649 nm).Cy3可以通过配有标准的TRITC滤波器的荧光计\成像仪及显微镜检测.由于其固有的高消光系数,甚至用肉眼也可以很容易地检测到凝胶和溶液中的Cy3.
参考文献：
http://en. wikipedia.org/wiki/Cyanine
| Physical Appearance | A solid |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 590.15 |
| Formula | C34H40ClN3O4 |
| Solubility | ≥59 mg/mL in DMSO; insoluble in H2O; ≥25.3 mg/mL in EtOH with ultrasonic |
| Chemical Name | (2,5-dioxopyrrolidin-1-yl) 6-[(2E)-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethylindol-1-ium-2-yl)prop-2-enylidene]indol-1-yl]hexanoate |
| SDF | Download SDF |
| Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)ON5C(=O)CCC5=O)(C)C)C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 最大激发光波 (nm) | 555 | 最大发射光波 (nm) | 570 |
| 消光系数 (M-1cm-1) | 150000 | 量子产量 | 0.31 |
| CF260 | 0.04 | CF280 | 0.09 |
质量控制和MSDS
- 批次:
化学结构
相关生物数据
