Sulfo-NHS-SS-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-NHS-SS-biotin是一种胺反应性的生物素化试剂。Sulfo-NHS-SS-biotin含有带负电荷的磺酸基，使其分子内产生足够的极性，可以直接加入到反应性水溶液中，而不需要提前溶解在有机溶剂中。尽管不需要提前溶解，sulfo-NHS-SS-biotin的水溶性原液必须快速制备并立即使用，以防止活性酯的水解。Sulfo-NHS-SS-biotin与含胺的蛋白或其它分子反应形成一个复合体，该复合体进一步与亲和素或链霉素探针相互作用，用亲和层析的方法纯化目标分子。
参考文献：
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
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• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
| Storage | Store at -20°C该产品在溶液中不稳定，建议现配现用 |
| M.Wt | 606.7 |
| Cas No. | 325143-98-4 |
| Formula | C19H27N4NaO9S4 |
| Synonyms | Biotin disulfide N-hydroxysulfosuccinimide ester |
| Solubility | ≥30.33 mg/mL in DMSO; <2.68 mg/mL in EtOH; <2.74 mg/mL in H2O |
| Chemical Name | sodium;1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | C1C(C(=O)N(C1=O)OC(=O)CCSSCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 生物素化方法[1]: | |
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样品 |
468/EV 细胞和468/uPAR 细胞 |
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溶解方法 |
可以溶于水中，为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。 |
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反应条件 |
1 mg/ml, 冰上15分钟 |
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应用 |
细胞悬液(1.5×106)用预冷的PBS洗三次后与1mg/mL sulfo-NHS-SS-biotin混合，在冰上放置15分钟。用含100 mmol/L谷氨酸的PBS能够终止生物素化反应。细胞经过PBS清洗后，用含蛋白酶抑制剂的RIPAh缓冲液（20 mmol/L sodium phosphate, 150 mmol/L NaCl (pH 7.4), 1% NP40, 0.1% SDS, and 0.5% deoxycholic acid）重悬。生物素化的膜蛋白用链霉亲和素琼脂糖拉下来，用SDS样品缓冲液洗脱出来，经过SDS-PAGE,电转到PVDF膜上, 最后用抗体进行杂交。 |
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References: [1]. Minji Jo, Boryana M. Eastman, Drue L. Webb, et al. Cell Signaling by Urokinase-type Plasminogen Activator Receptor Induces Stem Cell-like Properties in Breast Cancer cells . Cancer Res , 2010;70:8948-8958 |
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| 描述 | Sulfo-NHS-SS-Biotin是一种水溶性的生物素化试剂。 | |||||
| 靶点 | ||||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
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