HOBt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
羟基苯并三唑,缩写为HOBt,是作为苯并三唑衍生物的有机化合物。HOBt作为市售产品,是含有一些水(~11.7%水的HOBt一水合物晶体)的白色结晶粉末。它主要用于抑制单对映体手性分子的外消旋作用并提高肽合成的效率。自动肽合成包括被保护的氨基酸的氨基与活化酯的缩合。HOBt可用于制备活化酯,例如N-羟基琥珀酰亚胺酯。这些酯是不溶的,可以与胺在环境温度下反应得到酰胺[1]。HOBt还用于从除氨基酸之外的羧酸合成酰胺。这些底物不能转化为酰氯[2]。以这种方式可以制备离子抗生素的酰胺衍生物 [3]。
参考文献:
[1]. Knig W, Geiger R. Eine neue Methode zur Synthese von Peptiden: Aktivierung der Carboxylgruppe mit Dicyclohexylcarbodiimid unter Zusatz von 1‐Hydroxy‐benzotriazolen[J]. Chemische Berichte, 1970, 103(3): 788-798.
[2]. Myers A G, Yang B H, Chen H. Transformation of Pseudoephedrine Amides into Highly Enantiomerically Enriched Aldehydes, Alcohols, and Ketones[J]. Organic Syntheses, 2000: 29-29.
[3]. owicki D, Huczyński A, Ratajczak-Sitarz M, et al. Structural and antimicrobial studies of a new N-phenylamide of monensin A complex with sodium chloride[J]. Journal of Molecular Structure, 2009, 923(1): 53-59.
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Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 135.1 |
Cas No. | 2592-95-2 |
Formula | C6H5N3O |
Solubility | ≥22.4 mg/mL in EtOH with ultrasonic; ≥4.09 mg/mL in H2O with ultrasonic; ≥6.76 mg/mL in DMSO |
Chemical Name | 1-hydroxybenzotriazole |
SDF | Download SDF |
Canonical SMILES | C1=CC=C2C(=C1)N=NN2O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | HOBt是外消旋的抑制剂,用于多肽合成。 | |||||
Targets | racemization | |||||
IC50 |
质量控制和MSDS
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