EDC.HCl
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50值:N/A
EDC.HCl通常作为碳二亚胺试剂,是一种非常有用的工具,用于水介质中酰胺键(肽键)的形成。作为一种碳二亚胺试剂,它也有效地应用于氧化脱水、多核苷酸合成、酯化反应及内酯化反应。另外,EDC.HCl也广泛地用做水溶性冷凝剂[1]。
体外实验:碳二亚胺介导耦联的方法是一种最传统的肽键形成方法。为了合成目标化合物,充分混合羧酸和胺后,加入碳二亚胺试剂。反应完成后,碳二亚胺试剂变成相应的尿素[2]。最近,开发了一种简单快速的采用分光光度法流动注射分析的检测方法。该方法基于酸性水溶液中EDC.HCl与吡啶的反应。40C下反应一段时间后,测得400 nm下的吸光度。校准曲线在0到10%的EDC·HCl溶液范围内呈良好的线性关系,回归方程为y = 3.15 × 104x (x指EDC·HCl的百分比浓度; y指峰面积)。EDC·HCl在水中反应后,该方法用来监测其浓度[1]。
体内实验:到目前为止,没有体内数据。
临床试验:到目前为止,还未进行临床试验。
参考文献:
[1]Kunihiko Seno, Kazuki Matumura, Mitsuko Oshima, and Shoji Motomizu. Spectrophotometric determination of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr; 24: 505-8.
[2] Jad YE, Khattab SN, Beatriz G. Torre DL, Govender T, Kruger HG, Faham AE and Albericio F. EDC·HCl and potassium salts of oxyma and oxyma-B as superior coupling cocktails for peptide synthesis. Eur J Org Chem. 2015 May; 2015(14): 3116-20.
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 191.7 |
Cas No. | 25952-53-8 |
Formula | C8H17N3·HCl |
Solubility | ≥19.2 mg/mL in DMSO; ≥39 mg/mL in H2O; ≥39.6 mg/mL in ETOH |
Chemical Name | 3-(ethyliminomethylideneamino)-N,N-dimethylpropan-1-amine;hydrochloride |
SDF | Download SDF |
Canonical SMILES | CCN=C=NCCCN(C)C.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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