Influenza Hemagglutinin (HA) Peptide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HA标签复合体由质粒DNA、转铁蛋白 - 聚赖氨酸和流感病毒血凝素HA-2亚基N端序列衍生的聚赖氨酸共轭肽共同组成,用于将荧光素酶或β-半乳糖苷酶标记的基因转移进K562细胞、HeLa细胞和BNL CL.2肝细胞。DNA复合体中HA共轭多肽的存在使得该复合体可以破坏膜结构,使转铁蛋白 - 聚赖氨酸介导基因转移进入细胞[1]。
在流感病毒中,血凝素(HA)蛋白介导病毒与细胞表面的结合以及随后的病毒与细胞膜的融合。HA由受体结合亚单位HA1和膜融合亚单位HA2组成。病毒表面存在的HA1yHA2复合体是无活性的,膜融合是由HA蛋白的构象状态所调控的。当细胞内体处于酸性环境时,HA蛋白从天然构象(非膜融合)转换成融合活性(膜融合)的构象。HA的天然结构处于一种亚稳定的状态,对天然结构进行去稳定化后得到膜融合构象[2]。
参考文献:
1. E. WAGNER, C. PLANK et al, Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle. Proc. Nati. Acad. Sci. USA Vol. 89, pp. 7934-7938, September 1992
2. C. M. CARR, C. CHAUDHRY, P. S. KIM et al. Influenza hemagglutinin is spring-loaded by a metastable native conformation. Proc. Natl. Acad. Sci. USA Vol. 94, pp. 14306–14313
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Storage | Desiccate at -20°C |
M.Wt | 1102.15 |
Cas No. | 92000-76-5 |
Formula | C53H67N9O17 |
Synonyms | H-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-OH |
Solubility | ≥55.1 mg/mL in DMSO; ≥100.4 mg/mL in EtOH; ≥46.2 mg/mL in H2O |
Chemical Name | Influenza Hemagglutinin (HA) Peptide |
SDF | Download SDF |
Canonical SMILES | CC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC(CC2=CC=C(C=C2)O)C(=O)NC(C)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C4CCCN4C(=O)C(CC5=CC=C(C=C5)O)N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
描述 | Influenza Hemagglutinin (HA) Peptide是从流感病毒血凝素蛋白的表位衍生出的标签肽。 | |||||
靶点 | anti-HA-tag antibody | |||||
IC50 |
质量控制和MSDS
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