Cyclophosphamide monohydrate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50:作用于乙酰胆碱酯酶(AChE)的IC50值为511 μM。
Cyclophosphamide monohydrate用于儿童癌症治疗。作为前药,Cyclophosphamide monohydrate被细胞色素P-450酶代谢以产生活性烷基化类,导致其细胞毒性作用。
体外:Cyclophosphamide可逆性地抑制AChE,IC50值为511 μM。在控制系统中,作用于AChE 的Km值为132 μM,在CP处理系统中增加了78%。Cyclophosphamide的性质是线性混合型(部分竞争性和纯非竞争性)。估计的Ki和KI分别为378和582 μM,这表明非竞争性抑制相比竞争性抑制更具优势[1]。
体内:小鼠PK研究表明,四氯化碳预处理后cyclophosphamide血浆清除延迟。总烷化剂活性和4-羟基环磷酰胺的血浆水平上升更慢,达到较低的峰值。然而,在AUC中,血浆总烷基化活性或血浆4-羟基环磷酰胺在两组之间没有差别。因此,肿瘤细胞长期接触4-羟基环磷酰胺可能与四氯化碳预处理后cyclophosphamide的抗肿瘤活性增加有关 [2]。
临床试验:选择二十二个未接受已知影响药物代谢的其它治疗的儿童,其中有9名接受联合治疗,13例确定为对照。结果表明,接受氟康唑联合用药的患者血浆cyclophosphamide清除率较低[3]。
参考文献:
[1] al-Jafari AA,Duhaiman AS,Kamal MA. Inhibition of human acetylcholinesterase by cyclophosphamide. Toxicology.1995 Jan 19;96(1):1-6.
[2] Harris RN,Basseches PJ,Appel PL,Durski AM,Powis G. Carbon tetrachloride-induced increase in the antitumor activity of cyclophosphamide in mice: a pharmacokinetic study. Cancer Chemother Pharmacol.1984;12(3):167-72.
[3] Yule SM,Walker D,Cole M,μMcSorley L,Cholerton S,Daly AK,Pearson AD,Boddy AV. The effect of fluconazole on cyclophosphamide metabolism in children. Drug Metab Dispos.1999 Mar;27(3):417-21.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 279.1 |
Cas No. | 6055-19-2 |
Formula | C7H15Cl2N2O2P·H2O |
Solubility | ≥13.95 mg/mL in DMSO; ≥51.5 mg/mL in H2O with gentle warming and ultrasonic; ≥96 mg/mL in EtOH |
Chemical Name | N,N-bis(2-chloroethyl)-2-oxo-1,3,2λ5-oxazaphosphinan-2-amine;hydrate |
SDF | Download SDF |
Canonical SMILES | C1CNP(=O)(OC1)N(CCCl)CCCl.O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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