Scrambled 10Panx
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Scrambled 10Panx是10Panx (WRQAAFVDSY)的重排形式,抑制巨噬细胞对染料的吸收,而不影响细胞膜电流。10Panx是pannexin 1的模拟肽。10Panx在结构上与多肽PanxE1B相似,比PanxE1B少三个氨基酸,对卵母细胞中的pannexin1电流具有抑制效应,效果与PanxE1b相似。10Panx也能够阻断I2nd的激活,减少缓激肽(bradykinin)诱导的[Ca2+]i,减弱缓激肽诱导的quinacrine处理的人成纤维细胞中ATP的释放。
参考文献:
1. Roger J. Thompson, Michael F. Jackson, Michelle E. Olah, Ravi L. Rungta, Dustin J. Hines, Michael A. Beazely, John F. MacDonald, Brian A. MacVicar. Activation of Pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science 2008; 322: 1555-1559.
Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Marianna Certal, Cristina Costa, Maria Teresa 2. Magalhaes-Cardoso, Fatima Ferreirinha, Maria Adelina Costa and Paulo Correia-de-Sa. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Communication and Signaling 2013, 11:70
3. Junjie Wang, Meiyun Ma, Silviu Locovei, Robert W. Keane and Gerhard Dahl. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol 293: C1112-C1119, 2007
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1242.37 |
Cas No. | 1315378-72-3 |
Formula | C58H79N15O16 |
Synonyms | Scrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg |
Solubility | ≥31.05 mg/mL in DMSO |
SDF | Download SDF |
Canonical SMILES | CC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CC2=CNC3=CC=CC=C32)C(=O)NC(C)C(=O)NC(CCC(=O)N)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CO)NC(=O)C(CC4=CC=CC=C4)N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
培养的人皮下成纤维细胞 |
溶解方法 |
在无菌水中的溶解度为0.50 mg/ml。若配制更高浓度的溶液,一般步骤如下:请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月 |
反应条件 |
100 μM. |
实验结果 |
在培养的人皮下成纤维细胞中,10panx降低缓激肽诱导的[Ca2+]i流,减弱30 μM缓激肽诱导的ATP释放[1]。在卵母细胞中,10panx抑制pannexin1电流。在表达pannexin1的卵母细胞中,10panx减弱pannexin1通道的染料摄取[2]。 |
References: [1]. Pinheiro AR, Paramos-de-Carvalho D, Certal M, et al. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Cell Commun Signal, 2013, 11: 70. [2]. Wang J, Ma M, Locovei S, et al. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol. 2007, 293(3): C1112-1119. |
描述 | Scrambled 10Panx(Panx-1模拟抑制肽)是pannexin-1间隙连接的阻断剂。 | |||||
靶点 | Gap Channels | |||||
IC50 |
质量控制和MSDS
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