10Panx
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
10Panx是panx1模拟抑制肽,容易和可逆地抑制panx1电流。Panx1是在哺乳动物中异位表达的半通道蛋白。在难以有效和持续导入siRNA的细胞中,10Panx可以使研究者很容易和可逆地检测panx1的抑制,因而吸引了越来越多的关注。以前的研究表明,10Panx选择性地抑制P2X7R诱导的上染率以及ATP诱发的膜电流、持续上染率和半通道样电流,而对R2X7R激活的其它方面没有影响。半通道样电流是由panx1的过表达和ATP介导的IL-1β的释放诱导的。
参考文献:
Pablo Pelegrin and Annmarie Surprenant. Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptor. The EMBO Journal (2006) 25, 5071-5082
- 1. PusenWang, Baojie Shi1, et al. "Hepatic pannexin‐1 mediates ST2+ regulatory T cells promoting resolution of inflammation in lipopolysaccharide‐induced endotoxemia." Clin Transl Med. 2022 May;12(5):e849. PMID: 35593197
- 2. Shuzhen Dong, Kai Zhang, et al. "Carbenoxolone has the potential to ameliorate acute incision pain in rats." Mol Med Rep. 2021 Jul;24(1):520. PMID: 34013377
- 3. Fan J, Chen Y, et al. "Multi-walled carbon nanotubes induce IL-1β secretion by activating hemichannels-mediated ATP release in THP-1 macrophages." Nanotoxicology. 2020;1-18. PMID: 32538272
- 4. Wang Q, Li M, et al. "Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer." Theranostics. 2020;10(5):2141–2157. PMID: 32089737
- 5. Gao J, Zhang T, et al. "The F0F1 ATP synthase regulates human neutrophil migration through cytoplasmic proton extrusion coupled with ATP generation." Mol Immunol. 2017 Aug 23;90:219-226. PMID: 28843171
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1242.37 |
Cas No. | 955091-53-9 |
Formula | C58H79N15O16 |
Synonyms | 10Panx |
Solubility | ≥124.2 mg/mL in DMSO; insoluble in EtOH; ≥4.26 mg/mL in H2O with ultrasonic |
SDF | Download SDF |
Canonical SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC2=CC=CC=C2)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CCC(=O)N)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC3=CNC4=CC=CC=C43)N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
人红细胞(RBCs) |
溶解方法 |
在无菌水中的溶解度>10 mM。原液应分装,在-80℃以下可储存几个月。 |
反应条件 |
200 μM |
应用 |
10Panx可以减少90.9±15.5%的pO2降低诱导的ATP的释放,而不影响 ILO诱导的ATP的释放。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。 |
References: [1] Sridharan M, Adderley S, Bowles E, et al. Pannexin 1 (PANX1) is a Conduit for ATP Released from Human Red Blood Cells (RBCs) Exposed to Low Oxygen Tension. The FASEB Journal, 2010. |
描述 | 10Panx(Panx-1模拟抑制肽)是pannexin-1间隙连接的阻断剂。 | |||||
靶点 | Gap Channels | |||||
IC50 |
质量控制和MSDS
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