Geneticin, G-418 Sulfate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
G-418是80 S核糖体延伸的抑制剂[1]。
G418是一种氨基糖甙抗生素，对细菌、酵母、真菌、藻类、植物乃至动物细胞有广谱的抗性。1至300 g/ml的G418常用于原核和真核生物的克隆选择。临床分离的细菌菌株的抗生素耐药性实验发现，所有携带3'-O-氨基糖甙磷酸转移酶的菌株可耐受G418。在这些磷酸转移酶中，I类和II类分别由转座因子TnS和Tn601编码[2]。
除了选择功能外，G418对牛病毒性腹泻病毒（BVDV）也具有抗病毒活性。G418可通过剂量依赖的方式防止DENV-2感染BHK细胞导致的细胞病变效应（CPE），EC50值为3±0.4 g/ml[3]。
参考文献：
[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.
[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092.
[3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.
- 1. Qiyin Zhou, Wenyu Lin, et al. " Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells." Nat Commun. 2022 May 31;13(1):3034. PMID: 35641493
- 2. Zhang C, Duan Y, et al. "TFEB mediates immune evasion and resistance to mTOR inhibition of renal cell carcinoma via induction of PD-L1." Clin Cancer Res. 2019 Aug 5. pii: clincanres.0733.2019. PMID: 31383732
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 692.71 |
| Cas No. | 108321-42-2 |
| Formula | C20H40N4O10·2H2SO4 |
| Synonyms | Antibiotic G418 |
| Solubility | insoluble in EtOH; insoluble in DMSO; ≥64.6 mg/mL in H2O |
| Chemical Name | 2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid |
| SDF | Download SDF |
| Canonical SMILES | CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1, 2]: | |
|
细胞系 |
DENV-2转染的BHK细胞 |
|
溶解方法 |
该化合物可溶于H2O。若获取更高浓度的溶液，可在37℃下孵育10分钟，随后在超声波浴中摇匀。-20℃以下可储存数月。 |
|
反应条件 |
1–300 μg/ml，120 h |
|
应用 |
Geneticin（1-300 μg/ml）抑制多种原核及真核生物。Geneticin降低BHK细胞的活力，CC50值为165 ± 5 μg/ml。Geneticin预防DENV诱导的CPE，增加感染细胞的活力，EC50值大约为3 ± 0.4 μg/ml。Geneticin在72hpi后抑制DENV-2的病毒滴度的产量，EC50值为2 ± 0.1 μg/ml，EC90值约为20 ± 2 μg/ml。Geneticin抑制病毒产生的斑块数量，并显著减少剩余斑块的大小。 |
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注意事项 |
由于实验环境的不同，实际溶解度可能与理论值略有不同，请测试室内所有化合物的溶解度。 |
|
References: [1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7. [2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092. [3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27. |
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| Description | G-418是一种氨基糖甙抗生素。 | |||||
| 靶点 | 80 S ribosomes | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
