Q-VD-OPh
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
QVD-OPh是一种pan-caspase抑制剂,提供了一种低成本,无毒和高特异性的细胞凋亡抑制方式,并为抑制剂的设计提供了新视角。
Q-VD-OPh可以抑制重组的caspases 1、3、8和9,IC50值范围介于25-400 nM之间。Q-VD-OPh可以显著抑制放线菌素D诱导的细胞凋亡。与广泛使用的抑制剂ZVAD-fmk和Boc-D-fmk相比,Q-VD-OPh可以更加有效地抑制凋亡。Q-VD-OPh可以在不到4个小时的时间内完全抑制凋亡诱导子诱导的大量细胞的死亡。Q-VD-OPh也可以有效的阻止三大凋亡途径介导的细胞凋亡,分别是caspase 9/3、caspase 8/10和caspase 12。Q-VD-OPh具有更高的效力,但即使在高浓度下,它对细胞也是无毒的。Q-VD-OPh可以在不同的细胞类型和物种(人,小鼠和大鼠)中起作用,阻止终端caspase激活、底物切除以及与细胞凋亡相关的DNA片段的形成。
Q-VD-OPH作为一个独特的试剂,可以将难以复活的传代细胞从液氮中恢复过来。将Q-VD-OPH加入到要解冻的细胞中,细胞可以获得足够的时间从解冻的压力中恢复并开始增值,即使在标准的DMSO浓度(10%)下。Q-VD-OPh在溶液中可以稳定存在几个月,在培养基中有至少2.5天的效力。
参考文献:
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Storage | Store at -20°C |
M.Wt | 513.49 |
Cas No. | 1135695-98-5 |
Formula | C26H25F2N3O6 |
Solubility | ≥25.67 mg/mL in DMSO; insoluble in H2O; ≥28.75 mg/mL in EtOH |
Chemical Name | (S)-5-(2,6-difluorophenoxy)-3-((S)-3-methyl-2-(quinoline-2-carboxamido)butanamido)-4-oxopentanoic acid |
SDF | Download SDF |
Canonical SMILES | O=C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(COC1=C(F)C=CC=C1F)=O)=O)C2=NC3=CC=CC=C3C=C2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
JURL-MK1和HL60细胞 |
溶解方法 |
溶解于DMSO。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应时间 |
N/A |
应用 |
Q-VD-OPH在0.05 mM时可大幅度抑制caspase-3和caspase-7的活性,在低浓度时也可以抑制caspase-8。Q-VD-OPH在10 mM时抑制PARP-1的裂解,在2 mM时抑制DNA片段化,干扰细胞膜的功能。而药物诱导的细胞与纤连蛋白粘着性的丧失则需要10 mM Q-VD-OPh。 |
动物实验[2]: | |
动物模型 |
3个月大的TgCRND8小鼠 |
剂量 |
10 mg/kg,3次/周,3个月;腹腔注射 |
应用 |
在阿尔兹海默病的慢性治疗中,Q-VD-OPh抑制caspase-7的激活,限制tau和caspase裂解的病理变化。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。 |
References: 1. Ku?elová K1, Grebeňová D, Brodská B.Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes. J Cell Biochem. 2011 Nov;112(11):3334-42. 2. Rohn TT, Kokoulina P, Eaton CR et al. Caspase activation in transgenic mice with Alzheimer-like pathology: results from a pilot study utilizing the caspase inhibitor, Q-VD-OPh. Int J Clin Exp Med. 2009 Nov 5;2(4):300-8. |
Q-VD-OPH是一种脑和细胞通透性的、高效的和不可逆的pan-caspase抑制剂。作用于caspase-3、caspase-1、caspase-8和caspase-9,IC50值分别为25 nM、50 nM、100 nM和430 nM。 | ||||||
靶点 | caspase-1 | caspase-3 | caspase-8 | caspase-9 | ||
IC50 | 50nM | 25nm | 100nM | 430nM |
质量控制和MSDS
- 批次: