Amyloid Beta-Peptide (1-40) (human)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Amyloid β-Peptide (1-40) (human)是一个含有H2N-DAEFRHDSGYEVHHQKLVFFAE- DVGSNKGAIIGLMVGGVVIA-OH序列，分子量为4329.8的多肽。β淀粉样蛋白（Aβ或Abeta）是从淀粉样前体蛋白加工而成的含有36–43个氨基酸的多肽。Aβ是与阿尔兹海默病相关的淀粉样蛋白斑的成分。已有证据表明，Aβ是一个多功能肽，具有显著的非病理性活性[1]。Aβ是阿尔兹海默病患者脑中发现的沉积物的主要成分。在散发性阿尔兹海默病患者的脑中，Aβ的水平升高，造成脑血管病变和神经毒性[2]。Aβ蛋白是由β和γ分泌酶的连续作用而产生的。γ分泌酶产生Aβ肽的C末端，在APP的转膜结构域切割，可以产生许多36-43个氨基酸残基长度的异构体，最常见的异构体是Aβ40和Aβ42。更长形式的Aβ在内质网中切割产生，而更短形式的Aβ在反面高尔基网中产生[3]。
Figure1 structure of Amyloid β-Peptide (1-40) (human)
参考文献：
1. Lahiri DK, Maloney B (September 2010). "Beyond the signaling effect role of amyloid–β42 on the processing of AβPP, and its clinical implications". Exp. Neurol. 225 (1): 51–4.
2. Hardy J, Duff K, Hardy KG, Perez-Tur J, Hutton M (September 1998). "Genetic dissection of Alzheimer's disease and related dementias: amyloid and its relationship to tau". Nat. Neurosci. 1 (5): 355–8.
3. Hartmann T, Bieger SC, Brühl B, Tienari PJ, Ida N, Allsop D, Roberts GW, Masters CL, Dotti CG, Unsicker K, Beyreuther K (September 1997). "Distinct sites of intracellular production for Alzheimer's disease A beta40/42 amyloid peptides". Nat. Med. 3 (9): 1016–20.
- 1. Sijia Hao, Xia Li, et al. "Hydroxycinnamic Acid from Corncob and Its Structural Analogues Inhibit Aβ40 Fibrillation and Attenuate Aβ40-Induced Cytotoxicit." J. Agric. Food Chem. 2020,July 23, 2020 PMID:32700906
- 2. Hao S, Li X, et al. "CLVFFA-functionalized gold nanoclusters inhibit Aβ40 fibrillation, fibrils prolongation and disaggregate mature fibrils." ACS Chem Neurosci. 2019 Oct 22. PMID:31637909
- 3. Hald ES, Timm CD, et al. "Amyloid Beta Influences Vascular Smooth Muscle Contractility and Mechanoadaptation." J Biomech Eng. 2016 Nov 1;138(11). PMID:27590124
| Physical Appearance | A solid |
| Storage | Desiccate at -20°C |
| M.Wt | 4329.86 |
| Cas No. | 131438-79-4 |
| Formula | C194H295N53O58S |
| Synonyms | Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val |
| Solubility | insoluble in EtOH; ≥23.8 mg/mL in H2O; ≥43.28 mg/mL in DMSO |
| SDF | Download SDF |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]： | |
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细胞系 |
CA1区锥体细胞 |
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溶解方法 |
在无菌水中的溶解度 >10 mM。原液应分装，在-80℃以下可储存几个月。 |
|
反应条件 |
200 nM；20 min |
|
应用 |
在+20 mV的诱发电压下，Aβ（1-40）可逆地增加IBa，在11个细胞中的6个观察到该效果，并达到1.74 ± 0.06。激活曲线表明Aβ（1-40）引起明显的电压依赖的IBa增加，在0到+30 mV的测试电位下增强IBa。 |
| 动物实验[2]： | |
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动物模型 |
雄性查尔斯河Wistar大鼠 |
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剂量 |
400 mg/kg；腹腔注射 |
|
应用 |
在Aβ（1-40）注射后一周检测到统计学显著的基础ACh释放的减少（-30%），30天后，ACh释放的减少仍是统计学显著的（-38%）。K+刺激的乙酰胆碱的释放也同样受Aβ（1-40）影响，在损毁后第14天，Aβ（1-40）诱导ACh释放的显著下降（-43%）。 |
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注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。 |
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References: [1] Rovira C, Arbez N, Mariani J. Aβ (25–35) and Aβ (1–40) act on different calcium channels in CA1 hippocampal neurons. Biochemical and biophysical research communications, 2002, 296(5): 1317-1321. [2] Giovannelli L, Casamenti F, Scali C, et al. Differential effects of amyloid peptides β-(1–40) and β-(25–35) injections into the rat nucleus basalis. Neuroscience, 1995, 66(4): 781-792. |
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| Description | Aβ40是从淀粉样前体蛋白（APP）加工而成的多肽。 | |||||
| Targets | ||||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
