Laminin (925-933)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
层粘连蛋白（925-933）是一种来源于层粘连蛋白B1链925-933残基的多肽（Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg），与层粘连蛋白受体结合。
层粘连蛋白属于细胞外基质糖蛋白家族，是基底膜的主要非胶原成分。它们参与多种生物学过程，包括细胞粘着、分化、迁移、信号通路、突起生长和癌转移。层粘连蛋白由3条不同的链组成，分别为层粘连蛋白α、β和γ（以前分别称为A、B1和B2）。
层粘连蛋白β1链有7个结构上不同的区域，这是与其它β链异构体共有的部分。层粘连蛋白β1在大多数产生基底膜的组织中表达，是组成层粘连蛋白1的3条链之一。层粘连蛋白1是从Engelbreth-Holm-Swarm（EHS）肿瘤中分离到的第一个层粘连蛋白。β1链中参与细胞附着、趋化作用和与层粘连蛋白受体结合的序列已被鉴定到，其具有抑制癌转移的能力。
Figure1 Structure of Laminin
Figure2 Formula of LamininB1 (925-933)
参考文献：
1.Ikonen J, Pikkarainen T, Savolainen ER, Tryggvason K (Feb 1989). "A Hpa I polymorphism in the human laminin B1 chain gene on 7q22". Nucleic Acids Res 17 (1): 473.
2.Roche KB, Moore JW, Surana RB, Wilson BE (May 1989). "Aortic root dilatation associated with partial trisomy 7(q31.2----qter)". Pediatr Cardiol 10 (1): 53–5.
3.Bonneau D, Huret JL, Godeau G, Couet D, Putterman M, Tanzer J, Babin P, Larregue M (Sep 1991). "Recurrent ctb(7)(q31.3) and possible laminin involvement in a neonatal cutis laxa with a Marfan phenotype". Hum Genet 87 (3): 317–9.
- 1. Talbert, Lance Edward. "Advancing Academics Through Laboratory and Classroom Research: Probing Biomolecular Structure with Novel Mass Spectrometry Methods and Revisiting Student Learning Tools." UNIVERSITY OF CALIFORNIA. June 2019.
- 2. Lance E.Talbert, XingZhang, et al. "Synthesis of New S-S and C-C Bonds by Photoinitiated Radical Recombination Reactions in the Gas Phase." International Journal of Mass Spectrometry. Available online 5 April 2019.
- 3. Talbert LE, Julian RR. "Directed-Backbone Dissociation Following Bond-Specific Carbon-Sulfur UVPD at 213 nm." J Am Soc Mass Spectrom. 2018 Apr 5. PMID:29623659
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 967.06 |
| Cas No. | 110590-60-8 |
| Formula | C40H62N12O14S |
| Synonyms | H2N-Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-OH |
| Solubility | ≥15.53 mg/mL in H2O; ≥17.77 mg/mL in EtOH; ≥48.35 mg/mL in DMSO |
| Chemical Name | Laminin (925-933) |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C(C(=O)NCC(=O)NC(CO)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)CNC(=O)C2CCCN2C(=O)C(CC(=O)O)NC(=O)C(CS)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
HT-1080 and CHO cells, as well as B16F10 murine melanoma cells |
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Reaction Conditions |
HT-1080 and CHO cells, as well as B16F10 murine melanoma cells |
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Applications |
Laminin (925-933) stimulated the attachment of HT-1080 and CHO cells to culture plates when used at concentrations of 100 and 300 µg/ml for 1 h incubation. In addition, laminin (925-933) (100 µg/ml; 5 h incubation) was found to be a chemoattractant for the B16F10 murine melanoma cells, showing about 30% of the maximal response observed with full-length laminin. Laminin (925-933) could compete with full-length laminin and inhibit chemotactic response of the cells to full-length laminin. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Graf J, Iwamoto Y, Sasaki M, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell, 1987, 48(6): 989-996. |
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质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
