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Scrambled 10Panx

现货
Catalog No.
A2701
Panx-1模拟抑制肽,阻断pannexin-1间隙连接
组合的产品项目
规格价格库存 数量
1mg
¥ 500.00
现货
5mg
¥ 1,000.00
现货
10mg
¥ 1,800.00
现货
25mg
¥ 4,000.00
现货

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Background

Scrambled 10Panx is the scrambled form of 10Panx (WRQAAFVDSY), a mimetic peptide of pannexin 1 that inhibits dye uptake by macrophages without affecting cellular membrane currents. Being structurally similar to anther peptide PanxE1B, 10Panx is three amino acid shorter but exerting an inhibitory effect against pannexin1 currents in oocytes to a similar extent as PanxE1b. 10Panx is also able to block the activation of I2nd, decrease the bradykinin-induced [Ca2+]i response and attenuate bradykinin-induced ATP release from human fibroblasts loaded with quinacrine. However, in several studies, scrambled 10Panx has been proved to be less effective than 10Panx in its authentic form.

Reference

Roger J. Thompson, Michael F. Jackson, Michelle E. Olah, Ravi L. Rungta, Dustin J. Hines, Michael A. Beazely, John F. MacDonald, Brian A. MacVicar. Activation of Pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science 2008; 322: 1555-1559.

Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Marianna Certal, Cristina Costa, Maria Teresa Magalhaes-Cardoso, Fatima Ferreirinha, Maria Adelina Costa and Paulo Correia-de-Sa. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Communication and Signaling 2013, 11:70

Junjie Wang, Meiyun Ma, Silviu Locovei, Robert W. Keane and Gerhard Dahl. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol 293: C1112-C1119, 2007

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt1242.37
Cas No.1315378-72-3
FormulaC58H79N15O16
SynonymsScrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg
Solubility≥31.05mg/mL in DMSO
SDFDownload SDF
Canonical SMILESCC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CC2=CNC3=CC=CC=C32)C(=O)NC(C)C(=O)NC(CCC(=O)N)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CO)NC(=O)C(CC4=CC=CC=C4)N
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验[1]:

细胞系

培养的人皮下成纤维细胞

溶解方法

在无菌水中的溶解度为0.50 mg/ml。若配制更高浓度的溶液,一般步骤如下:请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月

反应条件

100 μM.

实验结果

在培养的人皮下成纤维细胞中,10panx降低缓激肽诱导的[Ca2+]i流,减弱30 μM缓激肽诱导的ATP释放[1]。在卵母细胞中,10panx抑制pannexin1电流。在表达pannexin1的卵母细胞中,10panx减弱pannexin1通道的染料摄取[2]。

References:

[1]. Pinheiro AR, Paramos-de-Carvalho D, Certal M, et al. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Cell Commun Signal, 2013, 11: 70.

[2]. Wang J, Ma M, Locovei S, et al. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol. 2007, 293(3): C1112-1119.

生物活性

描述 Scrambled 10Panx(Panx-1模拟抑制肽)是pannexin-1间隙连接的阻断剂。
靶点 Gap Channels          
IC50            

质量控制

化学结构

Scrambled 10Panx