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RGD (Arg-Gly-Asp) Peptides

现货
Catalog No.
B3708
抑制整合素结合到RGD基序
组合的产品项目
规格价格库存 数量
10mg
¥ 1,020.00
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Background

Synthetic peptides containing the arginine-glycine-aspartate (RGD) were extensively used as inhibitors of integrin-ligand interactions in studies of cell adhesion, migration, growth and differentiation, since the RGD motif is an integrin-recognition motif found in many ligands.

In vitro: RGD peptide can induce apoptosis in the absence of signals and integrin-mediated cell clustering. Previous study demonstrates that RGD peptides promote apoptosis through activation of conformation changes enhancing pro-caspase-3 activation and autoprocessing [1].

In vivo: Anima study suggested that the RGD-4C-FITC-peptide bound to both endothelial and tumor cells in vivo and that peptide targeting should allow the delivery of therapeutic drugs to both endothelial and tumor cells [2].

Clinical trials: Currenlty no clinical data are available.

References:
[1] Nature.  1999 Feb 11;397(6719):534-9.
RGD peptides induce apoptosis by direct caspase-3 activation. 
Buckley CD1, Pilling D, Henriquez NV, Parsonage G, Threlfall K, Scheel-Toellner D, Simmons DL, Akbar AN, Lord JM, Salmon M. 
[2] Cancer Res.  2002 Sep 15;62(18):5139-43. Arginine-glycine-aspartic acid (RGD)-peptide binds to both tumor and tumor-endothelial cells in vivo.  Zitzmann S1, Ehemann V, Schwab M.  

文献引用

1. Lee HN, Bosompra OA, Coller HA. "RECK isoforms differentially regulate fibroblast migration by modulating tubulin post-translational modifications." Biochem Biophys Res Commun. 2019 Mar 5;510(2):211-218. PMID:30704758

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt346.34
Cas No.99896-85-2
FormulaC12H22N6O6
Solubility<0.69mg/mL in DMSO, ≥17.3mg/mL in H2O with gentle warming
SDFDownload SDF
Canonical SMILESC(CC(C(=O)NCC(=O)NC(CC(=O)O)C(=O)O)N)CN=C(N)N
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

肺成纤维细胞

制备方法

在DMSO中的溶解度有限。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。

反应条件

1 mM;24小时

实验结果

在肺成纤维细胞中,RGD肽诱导细胞凋亡和caspase-3活化。此外,在粘附和分离的细胞中,RGD肽促进pro-caspase-3切割。因此,在贴壁细胞与其底物分离之前,包含RGD的多肽也能诱导细胞凋亡。

References:

[1]. Buckley CD, Pilling D, Henriquez NV, Parsonage G, Threlfall K, Scheel-Toellner D, Simmons DL, Akbar AN, Lord JM, Salmon M. RGD peptides induce apoptosis by direct caspase-3 activation. Nature. 1999 Feb 11;397(6719):534-9.

生物活性

描述 RGD是整合素-配体相互作用的抑制剂。
靶点 integrin-ligand interaction          
IC50            

质量控制

化学结构

RGD (Arg-Gly-Asp) Peptides

相关生物数据

Cilengitide