Retapamulin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Retapamulin是一种截短侧耳素抗生素[1].
Retapamulin被开发用于主要由革兰氏阳性菌\β溶血性链球菌和凝固酶阴性葡萄球菌引起的皮肤感染的局部治疗.Retapamulin尤其用于耐药菌株.在体外研究中,retapamulin具有有效的广谱活性.该物质对金黄色葡萄球菌\表皮葡萄球菌\腐生葡萄球菌\化脓性链球菌和无乳链球菌以及草绿色链球菌具有良好的抗菌活性,MIC50值分别为0.06 μg/ml\0.06 μg/ml\0.12 μg/ml\0.008 μg/ml\0.016 μg/ml和0.03 μg/ml.Retapamulin通过结合细菌核糖体的特定位点表现出抗菌作用[1,2].
参考文献：
[1] Jones R N, Fritsche T R, Sader H S, et al. Activity of retapamulin (SB-275833), a novel pleuromutilin, against selected resistant gram-positive cocci. Antimicrobial agents and chemotherapy, 2006, 50(7): 2583-2586.
[2] Rittenhouse S, Biswas S, Broskey J, et al. Selection of retapamulin, a novel pleuromutilin for topical use. Antimicrobial agents and chemotherapy, 2006, 50(11): 3882-3885.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 517.76 |
| Cas No. | 224452-66-8 |
| Formula | C30H47NO4S |
| Solubility | insoluble in H2O; ≥114.8 mg/mL in EtOH; ≥16.15 mg/mL in DMSO |
| Chemical Name | (3aR,4R,5R,7S,8S,9R,9aS,12R)-8-hydroxy-4,7,9,12-tetramethyl-3-oxo-7-vinyldecahydro-4,9a-propanocyclopenta[8]annulen-5-yl 2-(((1R,5S)-8-methyl-8-azabicyclo[3.2.1]octan-3-yl)thio)acetate |
| SDF | Download SDF |
| Canonical SMILES | O[C@H]([C@](C)(C=C)C1)[C@H](C)[C@]2(CCC3=O)[C@]3([H])[C@@]([C@@H]1OC(CSC4C[C@](CC5)([H])N(C)[C@]5([H])C4)=O)(C)[C@H](C)CC2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]： | |
|
细胞系 |
厌氧细菌菌株 |
|
溶解方法 |
在DMSO中的溶解度≥16.15mg/mL。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
512 mg/L |
|
应用 |
Retapamulin (<2 mg/l)抑制37/52 (71%) 脆弱类杆菌菌株和85/87 (98%)其它革兰氏阴杆菌。Retapamulin对痤疮丙酸杆菌和厌氧革兰氏阳性球菌的作用比克林霉素，甲硝唑和头孢曲松更有效。 |
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References: [1]. Odou MF, Muller C, Calvet L, Dubreuil L. In vitro activity against anaerobes of retapamulin, a new topical antibiotic for treatment of skin infections. J Antimicrob Chemother. 2007 Apr;59(4):646-51. Epub 2007 Mar 9. PubMed PMID:17350985. |
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质量控制和MSDS
- 批次:
化学结构
