Pyrrolidinedithiocarbamate ammonium
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pyrrolidinedithiocarbamate Ammonium is a potent inhibitor of nuclear factor-κB (NF-κB) [1].
NF-κB is a protein complex that controls DNA transcription and cytokine production. It is not only an important factor in regulating inflammation and immune responses and cell survival, but also involved in the cell transformation and tumor formation [1].
In human intestinal epithelial cell line HT-29 induced by interleukin-1β (IL-1β), pretreatment of cells with Pyrrolidinedithiocarbamate Ammonium (3 ~ 1000 μM) dose-dependently attenuated interleukin-8 (IL-8) production. Furthermore, Pyrrolidinedithiocarbamate Ammonium (100 μM) suppressed the accumulation of IL-8 mRNA. Pyrrolidinedithiocarbamate Ammonium inhibited the activation of NF-κB, by suppressing both NF-κB DNA binding and NF-κB-dependent transcriptional activity [2].
In Sprague-Dawley rats pretreated with bacillus Calmette-Guérin (BCG), injection of Pyrrolidinedithiocarbamate Ammonium (50, 100, and 200 mg/kg) reversed hepatic injury stimulated by BCG in vivo. Moreover, Pyrrolidinedithiocarbamate Ammonium inhibited down-regulation of Cytochrome P450 2E1 (CYP2E1) in a dose dependent manner, with ED50 value of 76 mg/kg [1].
References:
[1]. Qin J D, Cao Z H, Li X F, et al. Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury. Pharmaceutical Biology, 2014, 52(11): 1460-1466.
[2]. Németh Z H, Deitch E A, Szabó C, et al. Pyrrolidinedithiocarbamate inhibits NF-kappaB activation and IL-8 production in intestinal epithelial cells. Immunology Letters, 2003, 85(1): 41-46.
- 1. Tong Wang, Qi Sun, et al. "A1 astrocytes activated by 2-Chloroethanol via the ROS-induced p38 MAPK/NF-κB and AP-1 pathways modulate microglia polarization." Research Square. April 12th, 2021.
- 1. Tong Wang, Qi Sun, et al. "A1 Reactive Astrocytes Activated by 2-chloroethanol Modulate M1 Microglia Polarization through Upregulating IL-1β and TNF-α." Research Square. April 13th, 2021
- 3. HangSun, YufengZhou, et al. "Metformin protects 5-Fu-induced chemotherapy oral mucositis by reducing endoplasmic reticulum stress in mice." Eur J Pharm Sci. 2022 Jun 1;173:106182. PMID: 35405270
- 4. Li Yuan, Lingming Zhang, et al. "Upregulation of UGT1A1 expression by ursolic acid and oleanolic acid via the inhibition of the PKC/NF-κB signaling pathway." Phytomedicine. 2021 Nov;92:153726. PMID: 34536821
- 5. Zhao C, Zhou J, et al. "DHA Sensor GPR120 in Host Defense Exhibits the Dual Characteristics of Regulating Dendritic Cell Function and Skewing the Balance of Th17/Tregs." Int J Biol Sci. 2020 Jan 1;16(3):374-387. PMID: 32015675
- 6. Zhang ZL, Jiang QC, Wang SR. "Schisandrin A reverses doxorubicin-resistant human breast cancer cell line by the inhibition of P65 and Stat3 phosphorylation." Breast Cancer. 2017 Nov 27. PMID: 29181822
Physical Appearance | A solid |
Storage | Store at RT |
M.Wt | 164.28 |
Cas No. | 5108-96-3 |
Formula | C5H12N2S2 |
Synonyms | PDTC |
Solubility | insoluble in H2O; ≥12.975 mg/mL in EtOH with gentle warming; ≥7.3 mg/mL in DMSO |
Chemical Name | ammonium pyrrolidine-1-carbodithioate |
SDF | Download SDF |
Canonical SMILES | [S-]C(N1CCCC1)=S.[NH4+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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