Pyroxamide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pyroxamine是组蛋白去乙酰化酶的抑制剂[1]。
去乙酰化酶(HADC)可以除去组蛋白中ε-N-乙酰赖氨酸上的乙酰基,使组蛋白更紧密地包裹住DNA,阻止转录。
在小鼠红白血病细胞(MEL)中,pyroxamide通过细胞凋亡或细胞周期阻滞引起终端分化并抑制细胞生长。另外,pyroxamide增加乙酰化组蛋白H2A、H2B、H3和H4的水平。Pyroxamide抑制HDAC1活性,ID50值为100 nM[1]。在RD(胚胎型)和RH30B细胞系中,1.25-20.0 μM 的pyroxamide诱导细胞凋亡和乙酰化组蛋白的积累。此外,pyroxamide诱导p21/WAF1蛋白的表达,增加亚G1期细胞百分比[2]。
在人CWR22前列腺癌异种移植小鼠中,100或200 mg/kg 剂量的pyroxamide以剂量依赖性方式显著抑制肿瘤生长,增加p21/WAF1蛋白的表达[1]。
参考文献:
[1]. Butler LM, Webb Y, Agus DB, et al. Inhibition of transformed cell growth and induction of cellular differentiation by pyroxamide, an inhibitor of histone deacetylase. Clin Cancer Res, 2001, 7(4): 962-970.
[2]. Kutko MC, Glick RD, Butler LM, et al. Histone deacetylase inhibitors induce growth suppression and cell death in human rhabdomyosarcoma in vitro. Clin Cancer Res, 2003, 9(15): 5749-5755.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 265.31 |
Cas No. | 382180-17-8 |
Formula | C13H19N3O3 |
Solubility | Soluble in DMSO |
Chemical Name | N'-hydroxy-N-pyridin-3-yloctanediamide |
SDF | Download SDF |
Canonical SMILES | C1=CC(=CN=C1)NC(=O)CCCCCCC(=O)NO |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |