Pyrintegrin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pyrintegrin, a 2,4-disubstituted pyrimidine, is a novel small-molecule inhibitor of BMP signaling which previously found to promote human embryonic stem cells survival [1].
Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily of secreted signaling molecules with important roles in many biological contexts. BMPs could bind to specificserine/threonine kinase receptors and transduce the signal to the nucleus through Smad proteins [2].
In vitro: Pyrintegrin induced differentiation of hADSCs into adipogenic‐like cells in vitro. Pyrintegrin drug treatment can accelerate adipocyte differentiation and augment lipid accumulation, adiponectin and fatty acid secretion in adipogenic medium differentiated human adipose derived stem cells [1]. Pyrimidine (2 μM) enhanced the survival of hESC more than 30-fold after trypsin-mediated dissociation. Pyrimidine increased integrin-dependent attachment of hESC to extracellular matrices without significantly impacting cell proliferation. Pyrintegrin increased the binding of the activated β1 integrin-specific antibody HUTS-21 and enhanced the phosphorylation of multiple growth factor receptors and their downstream kinases, PI3K and MAPK [3].
In vivo: Pyrintegrin promoted proliferation and differentiation of adipose precursor cells depending on their microenvironment. Pyrintegrin increased the number of preadipocytes and the rate of adipocyte differentiation, resulting in enhanced de novo adipogenesis [1].
References:
[1] Shah B S. Pyrintegrin Induced Adipogenesis: Biology[D]. Columbia University, 2012.
[2] von Bubnoff A, Cho K W Y. Intracellular BMP signaling regulation in vertebrates: pathway or network[J]. Developmental biology, 2001, 239(1): 1-14.
[3] Xu Y, Zhu X, Hahm H S, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules[J]. Proceedings of the National Academy of Sciences, 2010, 107(18): 8129-8134.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 451.5 |
Cas No. | 1228445-38-2 |
Formula | C23H25N5O3S |
Solubility | ≤1mg/ml in ethanol;30mg/ml in DMSO;50mg/ml in dimethyl formamide |
Chemical Name | N-(cyclopropylmethyl)-4-[[4-(3,4-dihydro-6-hydroxy-1(2H)-quinolinyl)-2-pyrimidinyl]amino]-benzenesulfonamide |
SDF | Download SDF |
Canonical SMILES | OC1=CC2=C(C=C1)N(C3=CC=NC(NC4=CC=C(S(NCC5CC5)(=O)=O)C=C4)=N3)CCC2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |