Pyoluteorin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pyoluteorin is a secondary metabolite produced by the biological control organism Pseudomonas fluorescens Pf-5, a rhizosphere bacterium. Pyoluteorin shows no toxicity to seed- and root-rotting plant pathogens. Pyoluteorin production is influenced by positive autoregulation. Addition of pyoluteorin to liquid cultures of Pf-5 enhanced pyoluteorin production.
Pyoluteorin functions as a signal molecule negatively regulating additional secondary metabolites produced by Pseudomonas [1]. The pyoluteorin contributes to the biological control of soilborne plant diseases by some strains of Pseudomonas fluorescens, including Pf-5. Pyoluteorin induces its own productionand represses the other’s production [2]. Depending on the plant, pyoluteorin production plays an important role in the suppression of damping-off by strain CHA0 without being a major mechanism in disease suppression [3].
References:
[1] Brodhagen M, Henkels M D, Loper J E. Positive autoregulation and signaling properties of pyoluteorin, an antibiotic produced by the biological control organism Pseudomonas fluorescens Pf-5[J]. Applied and environmental microbiology, 2004, 70(3): 1758-1766.
[2] Kidarsa T A, Goebel N C, Zabriskie T M, et al. Phloroglucinol mediates cross‐talk between the pyoluteorin and 2, 4‐diacetylphloroglucinol biosynthetic pathways in Pseudomonas fluorescens Pf‐5[J]. Molecular microbiology, 2011, 81(2): 395-414.
[3] Maurhofer M, Keel C, Haas D, et al. Pyoluteorin production byPseudomonas fluorescens strain CHA0 is involved in the suppression ofPythium damping-off of cress but not of cucumber[J]. European journal of plant pathology, 1994, 100(3): 221-232.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 272.1 |
Cas No. | 25683-07-2 |
Formula | C11H7Cl2NO3 |
Synonyms | NSC 143092 |
Solubility | Soluble in DMSO |
Chemical Name | (4,5-dichloro-1H-pyrrol-2-yl)(2,6-dihydroxyphenyl)-methanone |
SDF | Download SDF |
Canonical SMILES | OC1=CC=CC(O)=C1C(C2=CC(Cl)=C(Cl)N2)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
铜绿假单胞菌 |
溶解方法 |
在DMSO中是可溶的。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
0~12500 ng/ml |
应用 |
Pyoluteorin有助于荧光假单胞菌某些菌株(包括Pf-5)对土壤传播的植物病害进行生物防治。Pyoluteorin诱导其自身产生并抑制其他的产生。 |
References: [1]. Kidarsa TA, Goebel NC, Zabriskie TM, Loper JE. Phloroglucinol mediates cross-talk between the pyoluteorin and 2,4-diacetylphloroglucinol biosynthetic pathways in Pseudomonas fluorescens Pf-5. Mol Microbiol. 2011 Jul;81(2):395-414. doi: 10.1111/j.1365-2958.2011.07697.x. Epub 2011 May 26. PMID: 21564338. |