Psoralidin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Psoralidin is a dual inhibitor of COX-2 and 5-LOX. Psoralidin, a coumestan derivative isolated from seed of Psoralea corylifolia, has been widely used in traditional medicine for bleeding, pollakiuria, enuresis, vitiligo, and psoriasis. Psoralidin shows a variety of biological activities such as anti-cancer, anti-bacterial, anti-oxidant, anti-depressant activities and regulation of insulin signaling [1].
Psoralidin (100 μM) inhibited COX-2 in IR-irradiated normal lung fibroblasts in HFL-1 and MRC-5 cells [1]. Psoralidin significantly inhibited IR-induced NF-κB-luciferase activity. Psoralidin inhibited the IR-induced COX-2 expression and PGE2 production through regulation of PI3K/Akt and NF-κB pathway. Also, psoralidin blocked IR-induced LTB4 production through direct interaction with 5-lipoxygenase activating protein (FLAP) in 5-LOX pathway. Psoralidin significantly attenuated IR-induced fibroblast migration [1]. Psoralidin inhibited LPS-induced iNOS expression via repressing Syk-mediated activation of PI3K-IKK-IκB signaling pathways [2]. Psoralidin enhanced TRAIL-induced apoptosis in HeLa cells through increased expression of TRAIL-R2 death receptor and depolarization of mitochondrial membrane potential [3].
In male BALB/c strain mice (eight weeks, average weight 22–25 g), psoralidin (5 mg/kg) suppressed IR-induced expression of pro-inflammatory cytokines (TNF-α, TGF-β, IL-6 and IL-1 α/β) and ICAM-1 [1].
References:
[1] Yang H J, Youn H S, Seong K M, et al. Psoralidin, a dual inhibitor of COX-2 and 5-LOX, regulates ionizing radiation (IR)-induced pulmonary inflammation[J]. Biochemical pharmacology, 2011, 82(5): 524-534.
[2] Chiou W F, Don M J, Liao J F, et al. Psoralidin inhibits LPS-induced iNOS expression via repressing Syk-mediated activation of PI3K-IKK-IκB signaling pathways[J]. European journal of pharmacology, 2011, 650(1): 102-109.
[3] Bronikowska J, Szliszka E, Jaworska D, et al. The coumarin psoralidin enhances anticancer effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)[J]. Molecules, 2012, 17(6): 6449-6464.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 336.3 |
Cas No. | 18642-23-4 |
Formula | C20H16O5 |
Solubility | ≤14mg/ml in DMSO;14mg/ml in dimethyl formamide |
Chemical Name | 3,9-dihydroxy-2-(3-methyl-2-buten-1-yl)-6H-benzofuro[3,2-c][1]benzopyran-6-one |
SDF | Download SDF |
Canonical SMILES | OC1=CC(O2)=C(C=C1)C3=C2C4=C(OC3=O)C=C(O)C(C/C=C(C)/C)=C4 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |