PSI-6206
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PSI-6206(RO-2433,GS-331007)[β-D-2’-deoxy-2’-fluoro-2’-C-methyluridine],是PSI-6130[β-D-2’-deoxy-2’-fluoro-2’-C-methylcytidine]的脱氨基衍生物,在HCV亚基因组复制子系统中是丙型肝炎病毒(HCV)复制的有效抑制剂。在HCV亚基因组复制子系统中,PSI-6206本身对HCV的复制不具有抑制活性,然而,其三磷酸形式RO2433-TP是HCV聚合酶合成RNA的有效抑制剂。RO2433-TP不仅抑制HCV复制酶的RNA合成活性(IC50 = 1.19 uM),还抑制重组HCV Con1 NS58的RNA合成活性(IC50 = 0.52 uM,Ki = 0.141 uM),其是在HCV基因组负链3'端衍生的杂聚RNA模板上合成。
参考文献:
Han Ma, Wen-Rong Jiang, Nicole Robledo, Vincent Leveque, Samir Ali, Teresa Lara-Jamie, Mohammad Masjedizadeh, David B. Smith, Nick Cammack, Klaus Klumpp, and Julian Symons. Characterization of the metabolic ctivation of hepatitis C virus nucleoside inhibitor β-D-2’-deoxy-2’-fluoro-2’-C-methylcytidine (PSI-6130) and identification of a novel active 5’-triphosphate species. The Journal of Biological Chemistry 2007; 282(41): 29812-29820
- 1.Eyer L, Šmídková M, et al. "Structure-activity relationships of nucleoside analogues for inhibition of tick-borne encephalitis virus. Antiviral Res." 2016 Jul 28;133:119-129. PMID:27476046
- 2.Eyer L, Nencka R, et al. "Nucleoside inhibitors of Zika virus." J Infect Dis. 2016 May 27. pii:jiw226. PMID:27234417
- 3.Rower, Joseph E, et al. "Validation and application of an LC-MS/MS method to determine the concentrations of sofosbuvir anabolites in cells." Antimicrobial agents and chemotherapy (2015): AAC-01693. PMID:26416874
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 260.22 |
Cas No. | 863329-66-2 |
Formula | C10H13FN2O5 |
Solubility | ≥13.1 mg/mL in DMSO; ≥24.15 mg/mL in EtOH with ultrasonic; ≥48.7 mg/mL in H2O with ultrasonic |
Chemical Name | 1-[(2R,3R,4R,5R)-3-fluoro-4-hydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]pyrimidine-2,4-dione |
SDF | Download SDF |
Canonical SMILES | CC1(C(C(OC1N2C=CC(=O)NC2=O)CO)O)F |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
描述 | PSI-6206 (RO2433)是一种选择性的HCV RNA聚合酶抑制剂。 | |||||
靶点 | HCV | |||||
IC50 |
质量控制和MSDS
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