PQ 401
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PQ401是一种新型有效的IGF-IR抑制剂，IC50值为12 μM[1]。
在人MCF-7细胞中，PQ401抑制IGF-IR的自磷酸化，IC50值为12 μM，抑制分离的IGF-IR激酶结构域的自磷酸化，IC50值小于1 μM。此外，10 μM 的PQ401可抑制血清培养的乳腺癌细胞生长。PQ401可通过Akt抗凋亡途径，减少IGF-I介导的信号。15 μM的PQ401处理二十四小时可减少caspase介导的细胞凋亡[1]。在小三叉神经节神经元中，PQ401可阻断IGF-1诱导的A型K(+)电流（IA），其与电压依赖性失活的超极化位移有关[2]。
在体内，一周三次的PQ401治疗可降低植入小鼠的MCNeuA细胞的生长速率[1]。
参考文献：
[1]. Gable KL, Maddux BA, Penaranda C, Zavodovskaya M, Campbell MJ, Lobo M, Robinson L, Schow S, Kerner JA, Goldfine ID, Youngren JF. Diarylureas are small-molecule inhibitors of insulin-like growth factor I receptor signaling and breast cancer cell growth. Mol Cancer Ther. 2006 Apr;5(4):1079-86.
[2]. Wang H, Qin J, Gong S, Feng B, Zhang Y, Tao J. Insulin-like growth factor-1 receptor-mediated inhibition of A-type K(+) current induces sensory neuronal hyperexcitability through the phosphatidylinositol 3-kinase and extracellular signal-regulated kinase 1/2 pathways, independently of Akt. Endocrinology. 2014 Jan;155(1):168-79.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 341.79 |
| Cas No. | 196868-63-0 |
| Formula | C18H16ClN3O2 |
| Solubility | insoluble in H2O; ≥17.1 mg/mL in DMSO; ≥2.38 mg/mL in EtOH with ultrasonic |
| Chemical Name | 1-(5-chloro-2-methoxyphenyl)-3-(2-methylquinolin-4-yl)urea |
| SDF | Download SDF |
| Canonical SMILES | CC1=NC2=CC=CC=C2C(=C1)NC(=O)NC3=C(C=CC(=C3)Cl)OC |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | PQ 401是一种细胞渗透性的胰岛素样生长因子受体（IGF1R）抑制剂，IC50值为12 μM。 | |||||
| 靶点 | IGF1R | |||||
| IC50 | 12 μM | |||||
化学结构
